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MB Sample ID: SA004272
| Local Sample ID: | SBEP_Microbiome.022 |
| Subject ID: | SU000102 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | 129/SvJ |
| Age Or Age Range: | 6- to 8-week-old |
| Gender: | Female |
| Animal Animal Supplier: | Jackson Laboratories,Bar Harbor, ME |
| Animal Housing: | specific pathogen-free conditions in filter-top cages |
| Animal Feed: | food provided ad libitum |
| Animal Water: | sterile water |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Treatment:
| Treatment ID: | TR000103 |
| Treatment Summary: | Experimental: Infected with 1.6 x 10^8 CFU S. Typhimurium | mouse | Control: treated with equal volume saline solution |
| Treatment Protocol Comments: | A final inoculum of 1.6 x 10^8 CFU S. Typhimurium/mouse was delivered by oral gavage to 10 mice (two cages of five mice each = Salmonella-infected). An equal number of mock-infected animals (two cages of five mice each = control) were administered an equal volume of sterile saline. Our infecting dose (10^8 CFU/mouse) aimed to establish a persistent infection that would ensure observation of S. Typhimurium proteins in downstream analyses. |
| Treatment: | Biotic / Abiotic |
| Treatment Compound: | S. Typhimurium / Saline |
| Treatment Dose: | 1.6 x 10^8 CFU S. Typhimurium/mouse / equal volume saline solution |
| Treatment Vehicle: | Saline |
| Animal Fasting: | 14 h before orogastric inoculation |
| Animal Endp Euthanasia: | Carbon Dioxide Asphixiation followed by Cervical Dislocaton |
| Animal Endp Tissue Coll List: | Feces |
| Animal Endp Tissue Proc Method: | Homogenization |
