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MB Sample ID: SA008926
| Local Sample ID: | MKRP4 |
| Subject ID: | SU000183 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | FVB (wild type)|MKR (FVB background) |
| Age Or Age Range: | 12 week old|12 week old |
| Gender: | male|male |
| Animal Animal Supplier: | JAX lab|LeRoith laboratory |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000256 | AN000257 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Exactive | Thermo Exactive |
| Column | Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm) | Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH000181 |
| Chromatography Summary: | Untarged mix-mode method |
| Instrument Name: | Thermo Exactive |
| Column Name: | Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm) |
| Column Pressure: | 150 Bar (Max) |
| Column Temperature: | 45 |
| Flow Gradient: | 0-15 min -1% B, 15-19 min- gradient 1 to 99% B, 18-24 min- 99% B, 24-25 min-gradient 99% to 1% B, 25-30 min-1% B |
| Flow Rate: | 0.1 ml/min |
| Injection Temperature: | 4 C |
| Sample Injection: | 5 uL |
| Solvent A: | 10% acetonitrile/90% water; 10 mM ammonium acetate ; 10 mM acetic acid (positive) or 10 mM NH3.H2O (negative) |
| Solvent B: | 90% acetonitrile/10% water; 10 mM ammonium acetate; 10 mM acetic acid (positive) or 10 mM ammonia (negative) |
| Analytical Time: | 30 min |
| Capillary Voltage: | 3.5 kv (positive), 3.1 kv (negative) |
| Transferline Temperature: | 275C |
| Washing Buffer: | 10% Acetonitrile |
| Weak Wash Solvent Name: | 10% Acetonitrile |
| Weak Wash Volume: | 600 uL |
| Strong Wash Solvent Name: | 90% Acetonitrile |
| Strong Wash Volume: | 200 uL |
| Sample Loop Size: | 20 uL |
| Chromatography Type: | Reversed phase |
