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MB Sample ID: SA008926

Local Sample ID:MKRP4
Subject ID:SU000183
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:FVB (wild type)|MKR (FVB background)
Age Or Age Range:12 week old|12 week old
Animal Animal Supplier:JAX lab|LeRoith laboratory
Species Group:Mammal

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Combined analysis:

Analysis ID AN000256 AN000257
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Exactive Thermo Exactive
Column Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm) Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm)
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Units Peak area Peak area


Chromatography ID:CH000181
Chromatography Summary:Untarged mix-mode method
Instrument Name:Thermo Exactive
Column Name:Shodex ODP2 HP-4B 5um polymer (50 x 4.6mm)
Column Pressure:150 Bar (Max)
Column Temperature:45
Flow Gradient:0-15 min -1% B, 15-19 min- gradient 1 to 99% B, 18-24 min- 99% B, 24-25 min-gradient 99% to 1% B, 25-30 min-1% B
Flow Rate:0.1 ml/min
Injection Temperature:4 C
Sample Injection:5 uL
Solvent A:10% acetonitrile/90% water; 10 mM ammonium acetate ; 10 mM acetic acid (positive) or 10 mM NH3.H2O (negative)
Solvent B:90% acetonitrile/10% water; 10 mM ammonium acetate; 10 mM acetic acid (positive) or 10 mM ammonia (negative)
Analytical Time:30 min
Capillary Voltage:3.5 kv (positive), 3.1 kv (negative)
Transferline Temperature:275C
Washing Buffer:10% Acetonitrile
Weak Wash Solvent Name:10% Acetonitrile
Weak Wash Volume:600 uL
Strong Wash Solvent Name:90% Acetonitrile
Strong Wash Volume:200 uL
Sample Loop Size:20 uL
Chromatography Type:Reversed phase