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MB Sample ID: SA008926
| Local Sample ID: | MKRP4 |
| Subject ID: | SU000183 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | FVB (wild type)|MKR (FVB background) |
| Age Or Age Range: | 12 week old|12 week old |
| Gender: | male|male |
| Animal Animal Supplier: | JAX lab|LeRoith laboratory |
| Species Group: | Mammal |
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Sample Preparation:
| Sampleprep ID: | SP000183 |
| Sampleprep Summary: | Frozen cells were mixed with dry ice-cool 80% methanol (mass-spec grade) at a ratio of 30 ?L/million cells, and then quickly thawed on heat block set at 50 °C for 5 min. The suspension was then processed by three rounds of 1 min vortex at max speed, chilled briefly on dry ice. The mixture was incubated at 4°C for 1 hour before centrifuge at 20,000 x g for 20 min at 4 °C. The supernatant was stored at -20 °C and used as metabolite extract for LC-MS analysis. |
| Sampleprep Protocol Filename: | Protocol_Methods_metformin_Li.pdf |
| Processing Method: | Homogenization and Centrifugation |
| Processing Storage Conditions: | On ice |
| Extraction Method: | 80% Methanol |
| Extract Concentration Dilution: | 30 microliters solvent per million cells |
| Extract Storage: | -20°C |
| Cell Type: | bone marrow |
