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MB Sample ID: SA008926

Local Sample ID:MKRP4
Subject ID:SU000183
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:FVB (wild type)|MKR (FVB background)
Age Or Age Range:12 week old|12 week old
Animal Animal Supplier:JAX lab|LeRoith laboratory
Species Group:Mammal

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Sample Preparation:

Sampleprep ID:SP000183
Sampleprep Summary:Frozen cells were mixed with dry ice-cool 80% methanol (mass-spec grade) at a ratio of 30 ?L/million cells, and then quickly thawed on heat block set at 50 °C for 5 min. The suspension was then processed by three rounds of 1 min vortex at max speed, chilled briefly on dry ice. The mixture was incubated at 4°C for 1 hour before centrifuge at 20,000 x g for 20 min at 4 °C. The supernatant was stored at -20 °C and used as metabolite extract for LC-MS analysis.
Sampleprep Protocol Filename:Protocol_Methods_metformin_Li.pdf
Processing Method:Homogenization and Centrifugation
Processing Storage Conditions:On ice
Extraction Method:80% Methanol
Extract Concentration Dilution:30 microliters solvent per million cells
Extract Storage:-20°C
Cell Type:bone marrow