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MB Sample ID: SA022637
| Local Sample ID: | UNIFESP_lip_LF7 |
| Subject ID: | SU000466 |
| Subject Type: | Human patients (Women) |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | <= 37 years |
| Gender: | Female |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000696 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity I-Class |
| Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Synapt G2 S QTOF |
| Ion Mode | POSITIVE |
| Units | m/z intensity |
Chromatography:
| Chromatography ID: | CH000504 |
| Chromatography Summary: | Reversed-phase analysis was performed on a Waters Ultra Performance liquid chromatography Acquity I-Class system using an Acquity HSS T3 2.10 x 100 mm column (Waters, Milford, USA). The column was maintained at 55 °C. The lipid extracts were analyzed by UPLC-MSE by injecting 10 μl of each sample dissolved in 1 mL of water:acetonitrile:2-propanol (H2O:ACN:2-propanol; 1:1:2, v:v:v) (Merck, Darmstadt, Germany). The chromatographic run was carried out in gradient mode with a flow rate of 0.5 mL min-1 with initial composition of 50% solvent B (ACN:2-propanol; 10:90) in solvent A (H2O:ACN; 40:60, v:v + 10 mM ammonium acetate (NH4Ac) (Sigma-Aldrich, Saint Louis, USA), maintained for 0 to 0.5 min. The gradient composition was changed linearly from 50% to 100% solvent B between 0.5 to 4.5 minutes, returning to the initial composition of 50% B in 4.6 minutes, kept to 5.0 minutes. |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 55ºC |
| Flow Rate: | 0.5 mL.min-1 |
| Solvent A: | 40% water/60% acetonitrile; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/90% isopropanol |
| Chromatography Type: | Reversed phase |
