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MB Sample ID: SA022637
| Local Sample ID: | UNIFESP_lip_LF7 |
| Subject ID: | SU000466 |
| Subject Type: | Human patients (Women) |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | <= 37 years |
| Gender: | Female |
| Species Group: | Human |
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Sample Preparation:
| Sampleprep ID: | SP000473 |
| Sampleprep Summary: | After thawing, lipids and metabolites were extracted based on the Bligh and Dyer protocol [26]. Briefly, 50 µL of sample was placed in a microtube, and 125 μL of chloroform (CHCl3) (Merck-Serono, Darmstadt, Germany) and 250 μL of methanol (CH3OH) (Merck-Serono, Darmstadt, Germany) were added. The mixture was submitted to homogenization for 2 minutes. The polar and nonpolar phases were separated by the addition of 125 μL of CHCl3 and 100 μL of Milli-Q water. Then, the samples were centrifuged for 5 minutes at 1000 x g. The organic phase (bottom) containing the lipids was recovered and transferred to a clean microtube, which was left open overnight at room temperature until the complete solvent evaporation. |
| Extraction Method: | Modified Bligh and Dyer protocol |
