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MB Sample ID: SA032597
Local Sample ID: | WT-4 |
Subject ID: | SU000614 |
Subject Type: | Plant |
Subject Species: | Arabidopsis thaliana |
Taxonomy ID: | 3702 |
Genotype Strain: | Col-0 |
Age Or Age Range: | 12 Days after germination |
Weight Or Weight Range: | 50 mg |
Species Group: | Plants |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000906 |
---|---|
Analysis type | MS |
Chromatography type | HILIC |
Chromatography system | Agilent 1290 |
Column | SeQuant ZIC-pHILIC (100 x 2.1mm,3.5um) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | Peak area |
Chromatography:
Chromatography ID: | CH000643 |
Chromatography Summary: | Samples were resolved using HILIC column |
Instrument Name: | Agilent 1290 |
Column Name: | SeQuant ZIC-pHILIC (100 x 2.1mm,3.5um) |
Column Temperature: | 30C |
Flow Gradient: | 87% B for 5 minutes, decreased to 55% over 8 minutes and held for 4 minutes before returning to 87% over 3 minute |
Flow Rate: | 0.3 ml min-1 |
Internal Standard: | 13C6 phenylalanine internal standard |
Solvent A: | 100% water; 5 mM ammonium acetate, pH 4 |
Solvent B: | 90% acetonitrile/10% water; 0.1% formic acid |
Chromatography Type: | HILIC |