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MB Sample ID: SA093605
| Local Sample ID: | bovine_CL_LD_replicate3 |
| Subject ID: | SU001358 |
| Subject Type: | Mammal |
| Subject Species: | Bos taurus |
| Taxonomy ID: | 9913 |
| Gender: | Female |
| Animal Animal Supplier: | JBS Beef Plant 3435 Edward Babe Gomez Ave, Omaha, NE 68107 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002130 | AN002131 | AN002132 | AN002133 | AN002134 | AN002135 | AN002136 | AN002137 | AN002138 |
|---|---|---|---|---|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS | MS | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase | Reversed phase | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity | Waters Acquity |
| Column | Waters Acquity BEH C18 (50 x 1.2mm,1.7um) | Agilent Eclipse XBD C8 (50 x 4.6mm, 1.8um) | Agilent Eclipse XBD C8 (50 x 4.6mm, 1.8um) | Agilent Eclipse XBD C8 (50 x 4.6mm, 1.8um) | Agilent Eclipse XBD C8 (50 x 4.6mm, 1.8um) | Agilent Eclipse XBD C8 (50 x 4.6mm, 1.8um) | Agilent Eclipse XBD C8 (50 x 4.6mm, 1.8um) | Thermo Hypersil Gold C18 (50 x 1.2mm,1.7um) | Agilent Eclipse XDB-PLUS C18 (50 x 1.2mm, 1.8um) |
| MS Type | ESI | ESI | ESI | ESI | ESI | ESI | ESI | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE | POSITIVE | POSITIVE | POSITIVE |
| Units | nM | nM | nM | nM | nM | nM | nM | nM | nM |
Chromatography:
| Chromatography ID: | CH001562 |
| Chromatography Summary: | Molecular species were resolved by reversed-phase liquid chromatography in the presence of class and sub-class specific internal standard compounds added to each sample. Selectivity was further enhanced by scheduling the detection of each compound according to its elution from the high-performance liquid chromatography (HPLC) column, known as scheduled MRM (sMRM). |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (50 x 1.2mm,1.7um) |
| Internal Standard: | DG(28:0)-d5, DG(30:0)-d5, DG(32:0)-d5, DG(34:0)-d5, DG(38:0)-d5, DG(40:10)-d5, DG(40:8)-d5, DG(40:4)-d5, DG(40:0)-d5, TG(44:1)-d5, TG(48:1)-d5, TG(50:0)-d5, TG(51:1)-d5, TG(58:10)-d5, TG(58:7)-d5, TG(62:16)-d5, TG(60:1)-d5 |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001563 |
| Chromatography Summary: | Molecular species were resolved by reversed-phase liquid chromatography in the presence of class and sub-class specific internal standard compounds added to each sample. Selectivity was further enhanced by scheduling the detection of each compound according to its elution from the high-performance liquid chromatography (HPLC) column, known as scheduled MRM (sMRM). |
| Instrument Name: | Waters Acquity |
| Column Name: | Agilent Eclipse XBD C8 (50 x 4.6mm, 1.8um) |
| Internal Standard: | LPC(17:0), PC(37:4), LPE(17:1), PE(37:4) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001564 |
| Chromatography Summary: | Molecular species were resolved by reversed-phase liquid chromatography in the presence of class and sub-class specific internal standard compounds added to each sample. Selectivity was further enhanced by scheduling the detection of each compound according to its elution from the high-performance liquid chromatography (HPLC) column, known as scheduled MRM (sMRM). |
| Instrument Name: | Waters Acquity |
| Column Name: | Thermo Hypersil Gold C18 (50 x 1.2mm,1.7um) |
| Internal Standard: | C17 Sphingosine, C17 Sphinganine,Cer(d18:1/12:0), GlcCer(d18:1/12:0), LacCer(d18:1/12:0) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001565 |
| Chromatography Summary: | Molecular species were resolved by reversed-phase liquid chromatography in the presence of class and sub-class specific internal standard compounds added to each sample. Selectivity was further enhanced by scheduling the detection of each compound according to its elution from the high-performance liquid chromatography (HPLC) column, known as scheduled MRM (sMRM). |
| Instrument Name: | Waters Acquity |
| Column Name: | Agilent Eclipse XDB-PLUS C18 (50 x 1.2mm, 1.8um) |
| Chromatography Type: | Reversed phase |
