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MB Sample ID: SA093605
| Local Sample ID: | bovine_CL_LD_replicate3 |
| Subject ID: | SU001358 |
| Subject Type: | Mammal |
| Subject Species: | Bos taurus |
| Taxonomy ID: | 9913 |
| Gender: | Female |
| Animal Animal Supplier: | JBS Beef Plant 3435 Edward Babe Gomez Ave, Omaha, NE 68107 |
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Sample Preparation:
| Sampleprep ID: | SP001366 |
| Sampleprep Summary: | Lipids from CL tissue LDs (~250uL) were extracted using a standard Bligh and Dyer extraction protocol and then dried and sent to Avanti Polar Lipids for lipidomics analysis. Extracts were received as dried residues in glass vials and were immediately stored at -80 °C until analysis. Bligh, E. G., & Dyer, W. J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37(8), 911–917. https://doi.org/10.1139/o59-099 |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Bligh & Dyer, chloroform:methanol (1:2, v:v) |
| Extract Storage: | -80℃ |
| Sample Resuspension: | 1mL of chloroform:methanol (8:2, v/v) |
| Sample Derivatization: | N/A |
| Subcellular Location: | Lipid Droplet |
