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MB Sample ID: SA218536

Local Sample ID:D3_TS_A_Hilic_Neg
Subject ID:SU002369
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:61-91
Gender:Female

Select appropriate tab below to view additional metadata details:


Combined analysis:

Analysis ID AN003728 AN003729 AN003730 AN003731
Analysis type MS MS MS MS
Chromatography type HILIC HILIC Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish Thermo Vanquish Thermo Vanquish
Column Waters Acquity BEH Amide (150 x 2.1mm,1.7um) Waters Acquity BEH Amide (150 x 2.1mm,1.7um) Waters Acquity BEH Amide (150 x 2.1mm,1.7um) Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Obitrap ID-X Tribrid Obitrap ID-X Tribrid Obitrap ID-X Tribrid Obitrap ID-X Tribrid
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Area integration Area integration Area integration Area integration

Chromatography:

Chromatography ID:CH002761
Chromatography Summary:Hilic ESI+
Methods Filename:AB_Human_Bone_Chromatography.pdf
Instrument Name:Thermo Vanquish
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:45°C
Flow Gradient:95% B at T0 hold for 1.5 min, linear decrease to 50% B at 11 min, hold for 4 mins
Flow Rate:200 μL/min
Injection Temperature:300°C
Sample Injection:3μL
Solvent A:100% water; 0.1% formic acid; 10 mM ammonium formate
Solvent B:100% acetonitrile; 10 mM ammonium formate; 0.1% formic acid
Preconditioning:4.5 mins B
Chromatography Type:HILIC
  
Chromatography ID:CH002762
Chromatography Summary:Hilic ESI-
Methods Filename:AB_Human_Bone_Chromatography.pdf
Instrument Name:Thermo Vanquish
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:45°C
Flow Gradient:95% B at T0 hold for 1.5 min, linear decrease to 50% B at 11 min, hold for 4 mins
Flow Rate:200 μL/min
Injection Temperature:300°C
Sample Injection:5μL
Solvent A:100% water; 0.1% formic acid; 10 mM ammonium formate
Solvent B:100% acetonitrile; 10 mM ammonium formate; 0.1% formic acid
Preconditioning:4.5 mins B
Chromatography Type:HILIC
  
Chromatography ID:CH002763
Chromatography Summary:RP ESI+
Methods Filename:AB_Human_Bone_Chromatography.pdf
Instrument Name:Thermo Vanquish
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:45°C
Flow Gradient:60% B at T0 hold for 1.5 min, linear increase to 85% B at 7 min, increase to 95% B at 12.5 min and hold for 4.5 min
Flow Rate:200 μL/min
Injection Temperature:300°C
Sample Injection:3μL
Solvent A:40% water/60 % acetonitrile; 0.1% formic acid; 10 mM ammonium formate
Solvent B:90% isopropanol/10% acetonitrile; 0.1% formic acid; 10mM ammonium formate
Preconditioning:4.5 mins B
Chromatography Type:Reversed phase
  
Chromatography ID:CH002764
Chromatography Summary:RP ESI-
Methods Filename:AB_Human_Bone_Chromatography.pdf
Instrument Name:Thermo Vanquish
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:45°C
Flow Gradient:60% B at T0 hold for 1.5 min, linear increase to 85% B at 7 min, increase to 95% B at 12.5 min and hold for 4.5 min
Flow Rate:200 μL/min
Injection Temperature:300°C
Sample Injection:5μL
Solvent A:40% water/60 % acetonitrile; 0.1% ammonia; 10 mM ammonium formate
Solvent B:90% isopropanol/10% acetonitrile; 0.1% ammonia solution; 10mM ammonium formate
Preconditioning:4.5 mins B
Chromatography Type:Reversed phase
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