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MB Sample ID: SA218536
| Local Sample ID: | D3_TS_A_Hilic_Neg |
| Subject ID: | SU002369 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 61-91 |
| Gender: | Female |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003728 | AN003729 | AN003730 | AN003731 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish | Thermo Vanquish |
| Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Obitrap ID-X Tribrid | Obitrap ID-X Tribrid | Obitrap ID-X Tribrid | Obitrap ID-X Tribrid |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Area integration | Area integration | Area integration | Area integration |
Chromatography:
| Chromatography ID: | CH002761 |
| Chromatography Summary: | Hilic ESI+ |
| Methods Filename: | AB_Human_Bone_Chromatography.pdf |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 45°C |
| Flow Gradient: | 95% B at T0 hold for 1.5 min, linear decrease to 50% B at 11 min, hold for 4 mins |
| Flow Rate: | 200 μL/min |
| Injection Temperature: | 300°C |
| Sample Injection: | 3μL |
| Solvent A: | 100% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 100% acetonitrile; 10 mM ammonium formate; 0.1% formic acid |
| Preconditioning: | 4.5 mins B |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH002762 |
| Chromatography Summary: | Hilic ESI- |
| Methods Filename: | AB_Human_Bone_Chromatography.pdf |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 45°C |
| Flow Gradient: | 95% B at T0 hold for 1.5 min, linear decrease to 50% B at 11 min, hold for 4 mins |
| Flow Rate: | 200 μL/min |
| Injection Temperature: | 300°C |
| Sample Injection: | 5μL |
| Solvent A: | 100% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 100% acetonitrile; 10 mM ammonium formate; 0.1% formic acid |
| Preconditioning: | 4.5 mins B |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH002763 |
| Chromatography Summary: | RP ESI+ |
| Methods Filename: | AB_Human_Bone_Chromatography.pdf |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 45°C |
| Flow Gradient: | 60% B at T0 hold for 1.5 min, linear increase to 85% B at 7 min, increase to 95% B at 12.5 min and hold for 4.5 min |
| Flow Rate: | 200 μL/min |
| Injection Temperature: | 300°C |
| Sample Injection: | 3μL |
| Solvent A: | 40% water/60 % acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10mM ammonium formate |
| Preconditioning: | 4.5 mins B |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002764 |
| Chromatography Summary: | RP ESI- |
| Methods Filename: | AB_Human_Bone_Chromatography.pdf |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Column Temperature: | 45°C |
| Flow Gradient: | 60% B at T0 hold for 1.5 min, linear increase to 85% B at 7 min, increase to 95% B at 12.5 min and hold for 4.5 min |
| Flow Rate: | 200 μL/min |
| Injection Temperature: | 300°C |
| Sample Injection: | 5μL |
| Solvent A: | 40% water/60 % acetonitrile; 0.1% ammonia; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% ammonia solution; 10mM ammonium formate |
| Preconditioning: | 4.5 mins B |
| Chromatography Type: | Reversed phase |
