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MB Sample ID: SA229079
| Local Sample ID: | 1 |
| Subject ID: | SU002410 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Strain Details: | MOLM-13 cells expressing FKBPF36V-tagged SETD1A |
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Combined analysis:
| Analysis ID | AN003791 | AN003792 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent 7100 CE | Agilent 7100 CE |
| Column | COSMO(+) (chemically coated with cationic polymer) capillary (50um x 105cm total length) (Nacalai Tesque,Kyoto,Japan) | COSMO(+) (chemically coated with cationic polymer) capillary (50um x 105 cm total length) (Nacalai Tesque,Kyoto,Japan) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6230 TOF | Agilent 6230 TOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | fmol/cell | fmol/cell |
Chromatography:
| Chromatography ID: | CH002804 |
| Chromatography Summary: | For cationic metabolite analysis, separations were carried out in a fused silica capillary (50 µm i.d. x 100 cm total length) filled with 1 M formic acid as the electrolyte. Approximately 5 nL of sample solution were injected at 50 mbar for 5 sec and 30 kV of voltage was applied. The capillary temperature was maintained at 20 ºC and the sample tray was cooled below 5 ºC. Methanol-water (50 % v/v) containing 0.01 µM Hexakis(2,2-difluoroethoxy)phosphazene was delivered as the sheath liquid at 10 µL/min. |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | COSMO(+) (chemically coated with cationic polymer) capillary (50um x 105cm total length) (Nacalai Tesque,Kyoto,Japan) |
| Chromatography Type: | CE |
| Chromatography ID: | CH002805 |
| Chromatography Summary: | For anionic metabolite analysis, a commercially available COSMO(+) (chemically coated with cationic polymer) capillary (50 µm i.d. x 105 cm total length) (Nacalai Tesque, Kyoto, Japan) was used with a 50 mM ammonium acetate solution (pH 8.5) as the electrolyte. Sample solution (30 nL) was injected at 50 mbar for 30 sec and -30 kV of voltage was applied. Ammonium acetate (5 mM) in 50 % methanol-water (v/v) containing 0.01 µM Hexakis(2,2-difluoroethoxy)phosphazene was delivered as the sheath liquid at 10 µl/min. |
| Instrument Name: | Agilent 7100 CE |
| Column Name: | COSMO(+) (chemically coated with cationic polymer) capillary (50um x 105 cm total length) (Nacalai Tesque,Kyoto,Japan) |
| Chromatography Type: | CE |
