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MB Sample ID: SA229079
| Local Sample ID: | 1 |
| Subject ID: | SU002410 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Strain Details: | MOLM-13 cells expressing FKBPF36V-tagged SETD1A |
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Combined analysis:
| Analysis ID | AN003791 | AN003792 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | CE | CE |
| Chromatography system | Agilent 7100 CE | Agilent 7100 CE |
| Column | COSMO(+) (chemically coated with cationic polymer) capillary (50um x 105cm total length) (Nacalai Tesque,Kyoto,Japan) | COSMO(+) (chemically coated with cationic polymer) capillary (50um x 105 cm total length) (Nacalai Tesque,Kyoto,Japan) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6230 TOF | Agilent 6230 TOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | fmol/cell | fmol/cell |
MS:
| MS ID: | MS003533 |
| Analysis ID: | AN003791 |
| Instrument Name: | Agilent 6230 TOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Electrospray ionization (ESI)-time-of-flight mass spectrometry (TOFMS) was conducted in the positive ion mode and the capillary voltage was set at 4,000V. A flow rate of heated dry nitrogen gas (heater temperature 300 ºC) was maintained at 7 psig. In TOFMS, the fragmentor-, skimmer-, and Oct RFV voltage was set at 75 V, 50 V, and 500V, respectively. Automatic recalibration of each acquired spectrum was performed using reference masses of reference standards. The 13C isotopic ion of a protonated methanol dimer ([2MeOH+H]+, m/z 66.0631) and Hexakis(2,2-difluoroethoxy)phosphazene ([M+H]+, m/z 622.0290) provided the lock mass for exact mass measurements. |
| Ion Mode: | POSITIVE |
| MS ID: | MS003534 |
| Analysis ID: | AN003792 |
| Instrument Name: | Agilent 6230 TOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | ESI-TOFMS was conducted in the negative ion mode; the capillary voltage was set at 3,500 V. For TOFMS, the fragmentor-, skimmer-, and Oct RFV voltage was set at 100 V, 50 V, and 500 V, respectively. Automatic recalibration of each acquired spectrum was performed using reference masses of reference standards, i.e., 13C isotopic ion of deprotonated acetic acid dimer ([2CH3COOH-H]-, m/z 120.0384), and Hexakis + deprotonated acetic acid (m/z 680.03554) provided the lock mass for exact mass measurements. |
| Ion Mode: | NEGATIVE |
