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MB Sample ID: SA127008
| Local Sample ID: | 103 |
| Subject ID: | SU001586 |
| Subject Type: | Plant |
| Subject Species: | Thalassiosira pseudonana CCMP1335 |
| Taxonomy ID: | 296543 |
| Genotype Strain: | CCMP1335 |
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Sample Preparation:
| Sampleprep ID: | SP001594 |
| Sampleprep Summary: | Phytoplankton cells were removed from filters using a sonicator SLPe (Branson) in ultra-pure water (Millipore), concentrated by a lyophilizer (Labconco), and kept -80oC until further processing. The samples were mixed with 600 µL of 30 mmol L-1 sodium phosphate buffer (18 mmol L-1 NaHPO4, 12 mmol L-1, pH 7.4) and an internal standard of 2,2-dimethyl-2-silapentane-5-sulfonate-d6 (DSS, 1 mmol L-1), vortexed at 4oC for 5 minutes, and centrifuged at 20,800 rcf using an ultracentrifuge (Eppendorf) at 4oC for 10 minutes, and supernatants were transferred to 5-mm NMR tubes (Bruker). For the test of model prediction experiment, 300 µL of supernatants were further diluted with 250 µL of the buffer and transferred to NMR tubes. All the sample preparation steps were done on ice or in a cold room (4oC). |
| Sampleprep Protocol Filename: | uchimiya_20201018_4_Sample_preparation_protocol_UGA_phytoplankton_Oct2020.docx |
| Sampleprep Protocol Comments: | Sample preparation protocol is in 4_Sample preparation protocol_UGA_phytoplankton_Oct2020.docx |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Water |
| Extract Enrichment: | Lyophilization |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Sodium phosphate buffer |
