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MB Sample ID: SA165096
| Local Sample ID: | LP25_pre_CSF_M |
| Subject ID: | SU001857 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 25(IQR 22-31) |
| Gender: | Male and female |
| Human Race: | European |
| Human Medications: | None |
| Human Prescription Otc: | None |
| Human Smoking Status: | None |
| Human Alcohol Drug Use: | None |
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Sample Preparation:
| Sampleprep ID: | SP001863 |
| Sampleprep Summary: | The cerebrospinal fluid samples (90 µl) were mixed with 5 µl of commercial internal standards and 5 µl of custom-synthesized 13C labeled standards and then incubated for 10 min at room temperature to permit small molecules and vitamins in the internal standards to associate with binding proteins. The metabolites were extracted with 400 µl of cold (-20 °C) methanol: acetonitrile (50:50, v/v), yielding a final concentration of methanol: acetonitrile: water (40:40:20, v/v/v). The samples were then vortexed vigorously, incubated on crushed ice for 10 min, and then centrifuged at 16,000 g for 10 min at 4 °C. The supernatants containing the extracted metabolites and internal standards were transferred to labeled cryotubes and stored at -80 °C prior to LC-MS/MS. |
| Processing Storage Conditions: | On ice |
| Extract Cleanup: | None |
| Extract Storage: | -80℃ |
| Sample Resuspension: | None |
| Sample Derivatization: | None |
