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MB Sample ID: SA178408
Local Sample ID: | Bov2_2 |
Subject ID: | SU002005 |
Subject Type: | Fungi |
Subject Species: | Beauveria bassiana |
Genotype Strain: | GenBank: KU751847; KU751848 |
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Sample Preparation:
Sampleprep ID: | SP002011 |
Sampleprep Summary: | Extraction was performed in microtubes, from 200 mg of fungal macerate to which 1 mL of 6:2:2 methanol:chloroform:water ice-cold extraction solution was added. These extraction microtubes were vigorously vortexed and placed in an ultrasonic low-temperature bath at 20 Hz s-1 for 15 min. The samples were then centrifuged (Eppendorf, Germany) at 4°C for 10 min at 14,000 rpm. Then, the supernatant was filtered using a 0.22 μm Whatman® filter (Merck, Germany) and transferred to a chromatographic vial where the extracts were lyophilized (Thermo Fischer Scientific, MA, USA) until completely dry. Finally, the lyophilized samples were resuspended in 200 μL of extraction solution and aliquoted for use in the GC-MS and LC-MS/MS. |
Processing Storage Conditions: | -80℃ |
Extract Storage: | -80℃ |