Summary of Study ST001927

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001217. The data can be accessed directly via it's Project DOI: 10.21228/M8D693 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001927
Study TitleFungal consortium of two Beauveria bassiana strains increases their virulence, growth, and resistance to stress: a metabolomic approach.
Study TypeUntargeted Metabolomics
Study SummaryEntomopathogenic fungi have been successfully used to control agricultural pests. They infect insects by coming into direct contact with their cuticle or when feeding on contaminated leaves or fruits. After contact with the insect, the entomopathogenic fungus penetrates its body cavity, where it grows and colonizes it from within, causing its death The use of two or more microorganisms in a microbial consortium has been increasingly applied in the biological control of diseases and pests. Beauveria bassiana is one of the most widely studied fungal species in biological control, yet little is known about its role in fungal consortiums. In a previous study, our group found that a consortium formed by two strains of B. bassiana had significantly greater biocontrol potential against the polyphagous caterpillars Duponchelia fovealis (Lepidoptera: Crambidae) than either strain on its own. Despite recent developments and growing efforts to better understand fungal metabolism and metabolites, much remains unknown. Metabolomics therefore represents an important field for evaluating the metabolites produced or modified by an organism or its relationship with the environment. In the present study, we aim to use untargeted metabolomics with gas and liquid chromatography coupled to mass spectrometers (GC-MS and LC-MS/MS) to evaluate the metabolic alterations caused by the co-cultivation of these strains and to correlate the metabolites produced by this consortium with the increased mortality in D. fovealis observed previosly.
Institute
Universidade Federal do Paraná
DepartmentPatologia Básica
LaboratoryLaboratório de Microbiologia e Biologia Molecular
Last NameStuart
First NameAndressa
AddressAv. Cel. Francisco Heráclito dos Santos, 100, 81530000, Jardim das Américas, Curitiba, Paraná, Brasil
Emailandressa.katiski@gmail.com
Phone5541991922779
Submit Date2021-09-29
Num Groups3
Total Subjects15
Study CommentsTwo genetically distinct strains of B. bassiana (Bov 3 and Bov 2) were cultivated in Agar Sabouraud culture medium, both separately and co-cultivated to form a fungal consortium. The metabolomic analysis were performed at the Laboratório de Genética de Plantas Max Feffer facility of the Escola Superior de Agricultura Luiz de Queiroz of the Universidade de São Paulo (ESALQ/USP). Three reads of every biological replicate (five per treatment) were performed, generating fifteen readings for each treatment. Pools of metabolites from each group were created as a quality control.
Raw Data AvailableYes
Raw Data File Type(s)cdf, raw(Waters)
Analysis Type DetailGC/LC-MS
Release Date2022-10-03
Release Version1
Andressa Stuart Andressa Stuart
https://dx.doi.org/10.21228/M8D693
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR001217
Project DOI:doi: 10.21228/M8D693
Project Title:Fungal consortium of two Beauveria bassiana strains increases their virulence, growth, and resistance to stress: a metabolomic approach.
Project Type:Untargeted Metabolomics
Project Summary:Entomopathogenic fungi have been successfully used to control agricultural pests. They infect insects by coming into direct contact with their cuticle or when feeding on contaminated leaves or fruits. After contact with the insect, the entomopathogenic fungus penetrates its body cavity, where it grows and colonizes it from within, causing its death The use of two or more microorganisms in a microbial consortium has been increasingly applied in the biological control of diseases and pests. Beauveria bassiana is one of the most widely studied fungal species in biological control, yet little is known about its role in fungal consortiums. In a previous study, our group found that a consortium formed by two strains of B. bassiana had significantly greater biocontrol potential against the polyphagous caterpillars Duponchelia fovealis (Lepidoptera: Crambidae) than either strain on its own. Despite recent developments and growing efforts to better understand fungal metabolism and metabolites, much remains unknown. Metabolomics therefore represents an important field for evaluating the metabolites produced or modified by an organism or its relationship with the environment. In the present study, we aim to use untargeted metabolomics with gas and liquid chromatography coupled to mass spectrometers (GC-MS and LC-MS/MS) to evaluate the metabolic alterations caused by the co-cultivation of these strains and to correlate the metabolites produced by this consortium with the increased mortality in D. fovealis observed previously.
Institute:Universidade Federal do Paraná
Department:Patologia Básica
Laboratory:Laboratório de Microbiologia e Biologia Molecular
Last Name:Stuart
First Name:Andressa
Address:Av. Cel. Francisco Heráclito dos Santos, 100, 81530000, Jardim das Américas, Curitiba, Paraná, Brasil
Email:andressa.katiski@gmail.com
Phone:5541991922779
Project Comments:Two genetically distinct strains of B. bassiana (Bov 3 and Bov 2) were cultivated in Agar Sabouraud culture medium, both separately and co-cultivated to form a fungal consortium. The metabolomic analysis were performed at the Laboratório de Genética de Plantas Max Feffer facility of the Escola Superior de Agricultura Luiz de Queiroz of the Universidade de São Paulo (ESALQ/USP). Three reads of every biological replicate (five per treatment) were performed, generating fifteen readings for each treatment. Pools of metabolites from each group were created as a quality control.
Contributors:Jason Lee Furuie, Thais Regiani Cataldi, Rodrigo Makowiecky Stuart, Maria Aparecida Cassilha Zawadneak, Carlos Alberto Labate, Ida Chapaval Pimentel

Subject:

Subject ID:SU002005
Subject Type:Fungi
Subject Species:Beauveria bassiana
Genotype Strain:GenBank: KU751847; KU751848

Factors:

Subject type: Fungi; Subject species: Beauveria bassiana (Factor headings shown in green)

mb_sample_id local_sample_id Fungal species
SA178405Bov2_1Beauveria bassiana Strain Bov2
SA178406Bov2_5Beauveria bassiana Strain Bov2
SA178407Bov2_4Beauveria bassiana Strain Bov2
SA178408Bov2_2Beauveria bassiana Strain Bov2
SA178409Bov2_3Beauveria bassiana Strain Bov2
SA178410Bov3_4Beauveria bassiana Strain Bov3
SA178411Bov3_5Beauveria bassiana Strain Bov3
SA178412Bov3_3Beauveria bassiana Strain Bov3
SA178413Bov3_2Beauveria bassiana Strain Bov3
SA178414Bov3_1Beauveria bassiana Strain Bov3
SA178415Consortium_4Consortium formed by Bov2 and Bov3 strains
SA178416Consortium_5Consortium formed by Bov2 and Bov3 strains
SA178417Consortium_3Consortium formed by Bov2 and Bov3 strains
SA178418Consortium_1Consortium formed by Bov2 and Bov3 strains
SA178419Consortium_2Consortium formed by Bov2 and Bov3 strains
Showing results 1 to 15 of 15

Collection:

Collection ID:CO001998
Collection Summary:Two genetically distinct strains of Beauveria bassiana (Bov 3 and Bov 2) were cultivated both separately and co-cultivated to form a fungal consortium. After the colonies had grown, the mycelium of each treatment (Bov 2, Bov 3, and the fungal consortium) was scraped from the culture medium with a spatula and then macerated separately in liquid nitrogen (N2)
Collection Protocol Filename:MetaboliteExtraction
Sample Type:Fungal mycelium
Storage Conditions:-80℃

Treatment:

Treatment ID:TR002017
Treatment Summary:Two genetically distinct strains of B. bassiana (Bov 3 and Bov 2) were cultivated in Petri dishes containing Agar Sabouraud culture medium, both separately and co-cultivated to form a fungal consortium. The cultures were incubated in the dark in a biological oxygen demand (BOD) oven for 14 days at 28°C.

Sample Preparation:

Sampleprep ID:SP002011
Sampleprep Summary:Extraction was performed in microtubes, from 200 mg of fungal macerate to which 1 mL of 6:2:2 methanol:chloroform:water ice-cold extraction solution was added. These extraction microtubes were vigorously vortexed and placed in an ultrasonic low-temperature bath at 20 Hz s-1 for 15 min. The samples were then centrifuged (Eppendorf, Germany) at 4°C for 10 min at 14,000 rpm. Then, the supernatant was filtered using a 0.22 μm Whatman® filter (Merck, Germany) and transferred to a chromatographic vial where the extracts were lyophilized (Thermo Fischer Scientific, MA, USA) until completely dry. Finally, the lyophilized samples were resuspended in 200 μL of extraction solution and aliquoted for use in the GC-MS and LC-MS/MS.
Processing Storage Conditions:-80℃
Extract Storage:-80℃

Combined analysis:

Analysis ID AN003133 AN003134 AN003135
Analysis type MS MS MS
Chromatography type GC Reversed phase Reversed phase
Chromatography system Agilent 7890A Waters Acquity UPLC Waters Acquity UPLC
Column Agilent DB-5 (20m x 0.18mm, 0.18um); Restek RX-T 17 (0.9m x 0.10mm, 0.10um) Waters Acquity UPLC HSS (2.1 x 100mm, 1.7um) Waters Acquity UPLC HSS (2.1 x 100mm, 1.7um)
MS Type EI ESI ESI
MS instrument type GC x GC-TOF QTOF QTOF
MS instrument name Leco Pegasus 4D GCxGC TOF Waters Ultima QTOF Waters Ultima QTOF
Ion Mode UNSPECIFIED POSITIVE NEGATIVE
Units Relative intensity Relative intensity

Chromatography:

Chromatography ID:CH002315
Chromatography Summary:(.cdf)
Methods Filename:LCMSMS
Instrument Name:Agilent 7890A
Column Name:Agilent DB-5 (20m x 0.18mm, 0.18um); Restek RX-T 17 (0.9m x 0.10mm, 0.10um)
Column Temperature:70 - 320 ºC
Flow Rate:1 mL.min-1
Injection Temperature:280 ºC
Sample Injection:1 uL
Oven Temperature:70°C for 2 min, increasing by 15°C·min-1 until it reached 320°C and then held at this temperature for 4 min.
Chromatography Type:GC
  
Chromatography ID:CH002316
Chromatography Summary:Positive Mode (.raw)
Methods Filename:LCMSMS
Instrument Name:Waters Acquity UPLC
Column Name:Waters Acquity UPLC HSS (2.1 x 100mm, 1.7um)
Column Temperature:35 ºC
Flow Gradient:95% solvent A and 5% B. The gradient increased linearly to 75% A and 25% B over the next 6 min. The polarity was reversed to 25% A and 75% B for 6 min, and finally 5% A and 95% B for 1 min
Flow Rate:0.5 mL·min-1
Solvent A:Water with formic acid
Solvent B:Acetonitrile with formic acid.
Chromatography Type:Reversed phase
  
Chromatography ID:CH002317
Chromatography Summary:Negative mode (.raw)
Methods Filename:LCMSMS
Instrument Name:Waters Acquity UPLC
Column Name:Waters Acquity UPLC HSS (2.1 x 100mm, 1.7um)
Column Temperature:35 ºC
Flow Gradient:95% solvent A and 5% B. The gradient increased linearly to 75% A and 25% B over the next 6 min. The polarity was reversed to 25% A and 75% B for 6 min, and finally 5% A and 95% B for 1 min
Flow Rate:0.5 mL·min-1
Solvent A:Water with formic acid
Solvent B:Acetonitrile with formic acid.
Chromatography Type:Reversed phase

MS:

MS ID:MS002913
Analysis ID:AN003133
Instrument Name:Leco Pegasus 4D GCxGC TOF
Instrument Type:GC x GC-TOF
MS Type:EI
MS Comments:Data from GC-MS was processed using ChromaTOF 4.32 software. NIST library version 11 was used for the identification of metabolites.
Ion Mode:UNSPECIFIED
Ion Source Temperature:250 ºC
Ionization Energy:70 eV
  
MS ID:MS002914
Analysis ID:AN003134
Instrument Name:Waters Ultima QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Generated data were pre-processed using MassLynx 4.1 software
Ion Mode:POSITIVE
Capillary Voltage:3 kV
Source Temperature:150 ºC
Desolvation Gas Flow:550 L/hr.
  
MS ID:MS002915
Analysis ID:AN003135
Instrument Name:Waters Ultima QTOF
Instrument Type:QTOF
MS Type:ESI
MS Comments:Generated data were pre-processed using MassLynx 4.1 software
Ion Mode:NEGATIVE
Capillary Voltage:3 kV
Source Temperature:150 ºC
Desolvation Gas Flow:550 L/hr.
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