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MB Sample ID: SA004260

Local Sample ID:SBEP_Microbiome.004
Subject ID:SU000102
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:129/SvJ
Age Or Age Range:6- to 8-week-old
Animal Animal Supplier:Jackson Laboratories,Bar Harbor, ME
Animal Housing:specific pathogen-free conditions in filter-top cages
Animal Feed:food provided ad libitum
Animal Water:sterile water
Species Group:Mammal

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Treatment ID:TR000103
Treatment Summary:Experimental: Infected with 1.6 x 10^8 CFU S. Typhimurium | mouse | Control: treated with equal volume saline solution
Treatment Protocol Comments:A final inoculum of 1.6 x 10^8 CFU S. Typhimurium/mouse was delivered by oral gavage to 10 mice (two cages of five mice each = Salmonella-infected). An equal number of mock-infected animals (two cages of five mice each = control) were administered an equal volume of sterile saline. Our infecting dose (10^8 CFU/mouse) aimed to establish a persistent infection that would ensure observation of S. Typhimurium proteins in downstream analyses.
Treatment:Biotic / Abiotic
Treatment Compound:S. Typhimurium / Saline
Treatment Dose:1.6 x 10^8 CFU S. Typhimurium/mouse / equal volume saline solution
Treatment Vehicle:Saline
Animal Fasting:14 h before orogastric inoculation
Animal Endp Euthanasia:Carbon Dioxide Asphixiation followed by Cervical Dislocaton
Animal Endp Tissue Coll List:Feces
Animal Endp Tissue Proc Method:Homogenization