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MB Sample ID: SA127022
| Local Sample ID: | 25 |
| Subject ID: | SU001586 |
| Subject Type: | Plant |
| Subject Species: | Thalassiosira pseudonana CCMP1335 |
| Taxonomy ID: | 296543 |
| Genotype Strain: | CCMP1335 |
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Treatment:
| Treatment ID: | TR001601 |
| Treatment Summary: | Diel experiment – An axenic strain of marine diatom Thalassiosira pseudonana CCMP1335 was cultured at 18 oC in three replicate 15-L polycarbonate bottles containing 10 L of L1 medium in which NaH13CO3 (Cambridge Isotope Laboratories, CLM-441) was used for sodium bicarbonate. The light cycle consisted of 16 h light, during which light intensity varied gradually between 0 and 150 µmol photon m-2 s-1 with a maximum intensity at noon, followed by 8 h of dark. Bacterial strain Ruegeria pomeroyi DSS-3 was grown at 30oC on ½ YTSS agar and transferred to ½ YTSS liquid2 medium for overnight growth. Axenic T. pseudonana cultures grown for 6 days were inoculated with bacterial cells washed in L1 medium three times (final concentration, 106 bacterial cells mL-1). Co-cultures were pre-incubated for two days. Test of model prediction experiment – T. pseudonana was inoculated into L1 as described above. Triplicate samples were inoculated with three heterotrophic bacteria (R. pomeroyi DSS-3, Stenotrophomonas sp. SKA-14, and Polaribacter dokdonensis MED-152). Another set of triplicate samples was kept axenic (diatom only). The cultures were maintained under 16 h of light period (160 µmol photons m-2 s-1) and 8 h of dark period cycles. |
| Treatment Protocol Filename: | uchimiya_20201018_3_Treatment_protocol_UGA_phytoplankton_Oct2020.docx |
| Treatment Protocol Comments: | Treatment protocol is in 3_Treatment protocol_UGA_phytoplankton_Oct2020.docx |
| Treatment: | Test of model prediction experiment: with/without bacteria |
| Plant Light Period: | Light period: 16 hr, dark period: 8 hr |
| Plant Temp: | 18oC |
