Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA137924

Local Sample ID:	Fru3
Subject ID:	SU001699
Subject Type:	Cultured cells
Subject Species:	Mus musculus
Taxonomy ID:	10090
Gender:	Female

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Treatment:

Treatment ID:	TR001712
Treatment Summary:	For metabolic flux analysis of 13C-labeled glucose, intestinal organoids were pretreated with unlabeled fructose (10 mM) for 24 hr, washed with PBS, and then switched into 13C labeled glucose-containing DMEM/F12 medium (10 mM U-[13C] D-glucose, Cambridge Isotope Laboratories) for 6 hr. To trace the metabolism of fructose, intestinal organoids were cultured in unlabeled fructose-containing DMEM/F12 medium (10 mM D-fructose, 0 mM D-glucose, 2.5 mM L-glutamine) for 12 hr, and then switched into 13C labeled fructose-containing medium (10 mM U-[13C] D-fructose, Sigma-aldrich) for 6 hr.

Treatment ID:

TR001712

Treatment Summary:

For metabolic flux analysis of 13C-labeled glucose, intestinal organoids were pretreated with unlabeled fructose (10 mM) for 24 hr, washed with PBS, and then switched into 13C labeled glucose-containing DMEM/F12 medium (10 mM U-[13C] D-glucose, Cambridge Isotope Laboratories) for 6 hr. To trace the metabolism of fructose, intestinal organoids were cultured in unlabeled fructose-containing DMEM/F12 medium (10 mM D-fructose, 0 mM D-glucose, 2.5 mM L-glutamine) for 12 hr, and then switched into 13C labeled fructose-containing medium (10 mM U-[13C] D-fructose, Sigma-aldrich) for 6 hr.