Summary of study ST001622

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001041. The data can be accessed directly via it's Project DOI: 10.21228/M84M6M This work is supported by NIH grant, U2C- DK119886.


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Study IDST001622
Study TitleIsotope Tracing Analysis in Intestinal Organoids with fructose
Study SummaryWe isolated and cultured Intestinal Organoids from Mouse. To trace the metabolism of fructose, intestinal organoids were cultured in unlabeled fructose-containing DMEM/F12 medium (10 mM D-fructose, 0 mM D-glucose, 2.5 mM L-glutamine) for 12 hr, and then switched into 13C labeled fructose-containing medium (10 mM U-[13C] D-fructose, Sigma-aldrich) for 6 hr.
China Pharmaceutical University
DepartmentSchool of Medicine
Last NameHou
First NameYuanlong
Addresstongjiaxiang, nanjing, jiangsu, 210000, China
Submit Date2020-12-09
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2020-12-16
Release Version1
Yuanlong Hou Yuanlong Hou application/zip

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Project ID:PR001041
Project DOI:doi: 10.21228/M84M6M
Project Title:Dietary composition analysis studies
Project Type:Untargeted metabolomics analysis with two diets
Project Summary:Dietary patterns and psychosocial factors, ubiquitous part of modern lifestyle, critically shape the gut microbiota and human health. However, it remains obscure how dietary and psychosocial inputs coordinately modulate the gut microbiota and host impact. Here, we show that dietary raffinose metabolism to fructose couples stress-induced gut microbial remodeling to intestinal stem cells (ISC) renewal and epithelial homeostasis. Chow diet (CD) and purified diet (PD) confer distinct vulnerability to gut epithelial injury, microbial alternation and ISC dysfunction in chronically restrained mice. CD preferably enriches Lactobacillus reuteri, and its colonization is sufficient to rescue stress-triggered epithelial injury.
Institute:China Pharmaceutical University
Department:School of Medicine
Last Name:Yunalong
First Name:Hou
Address:Nanjing, Jiangsu