Summary of study ST000641

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000458. The data can be accessed directly via it's Project DOI: 10.21228/M8VG74 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000641
Study TitleTargeted Amino Acids in American Indian Adolescents (part II)
Study SummaryThe goal of this study is to determine the effect of physical activity on the blood concentrations of several compounds that are proposed to be markers of diabetic risk. We will compare results from normal weight and obese American Indian adolescents, with high or low habitual physical activity. We will also compare the same blood markers in the obese group before and after 4 months of exercise to measure the benefit of the program.
Institute
Mayo Clinic
Last NameShort
First NameKevin
AddressUniversity of Oklahoma Health Sciences Center 1200 Children’s Ave, Suite 4500 Oklahoma City, OK 73104
Emailkevin-short@ouhsc.edu
Phone405-271-8001
Submit Date2017-06-23
Analysis Type DetailLC-MS
Release Date2020-06-26
Release Version1
Kevin Short Kevin Short
https://dx.doi.org/10.21228/M8VG74
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000458
Project DOI:doi: 10.21228/M8VG74
Project Title:Biomarkers of diabetes risk in children: role of obesity and exercise
Project Summary:This proposal addresses a gap in knowledge about biomarkers for insulin resistance and diabetes. It remains unclear why some populations develop diabetes at higher rates than others and whether prevention and treatment strategies should also differ. The main goal of the current study is to determine the impact of body composition and physical activity on biomarkers for insulin resistance in American Indian adolescents. We propose to use a targeted metabolomic approach to measure amino compounds and fatty acids in plasma. We will address these goals by exploring two Specific Aims: 1. To determine the effects of obesity and physical activity on circulating biomarkers for diabetes and metabolic disease risk in American Indian youth. Plasma samples used for measurements of amino compounds and fatty acids will be acquired from boys and girls 11-21 years old classified as, a) obese, low habitual physical activity and low fitness; b) normal weight, low habitual physical activity and low fitness; or, c) normal weight, high habitual physical activity and high fitness. 2. To determine the impact of exercise training on circulating biomarkers for diabetes and metabolic disease risk in obese American Indian youth. The same obese group tested in the cross-sectional comparison in Aim 1 will complete 16 weeks of aerobic exercise training. Plasma samples acquired before and after training will be used to measure amino compounds and fatty acids. The results of this project will provide novel information about the metabolic changes associated with insulin resistance, obesity, and physical activity in young people who are at risk for, but have not yet developed cardiometabolic disease. The study design takes advantage of, and supports the scientific partnership among investigators at the University of Oklahoma Health Sciences Center, Choctaw Nation of Oklahoma, and Mayo Clinic Rochester. This will set the stage for future lifestyle intervention studies to further explore the concepts outlined in this proposal.
Institute:Mayo Clinic
Last Name:Short
First Name:Kevin
Address:University of Oklahoma Health Sciences Center 1200 Children’s Ave, Suite 4500 Oklahoma City, OK 73104
Email:kevin-short@ouhsc.edu
Phone:405-271-8001

Subject:

Subject ID:SU000664
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id date time group
SA036169ms6169-7610/10/2014 baseline Overweight/Obese adolescents 48 week excersise program
SA036170ms6169-1310/11/2013 baseline Overweight/Obese adolescents 48 week excersise program
SA036171ms6169-10510/15/2015 baseline normal weight reference group adolescents
SA036172ms6169-10410/19/2015 baseline normal weight reference group adolescents
SA036173ms6169-910/2/2013 baseline Overweight/Obese adolescents 48 week excersise program
SA036174ms6169-1510/2/2013 baseline Overweight/Obese adolescents 48 week excersise program
SA036175ms6169-6610/23/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036176ms6169-1810/25/2013 baseline Overweight/Obese adolescents 48 week excersise program
SA036177ms6169-5210/28/2015 post-training Overweight/Obese adolescents 48 week excersise program
SA036178ms6169-2710/30/2014 baseline Overweight/Obese adolescents 48 week excersise program
SA036179ms6169-1110/4/2013 baseline Overweight/Obese adolescents 48 week excersise program
SA036180ms6169-9010/5/2015 post-training Overweight/Obese adolescents 48 week excersise program
SA036181ms6169-6011/17/2015 post-training Overweight/Obese adolescents 48 week excersise program
SA036182ms6169-5811/23/2015 post-training Overweight/Obese adolescents 48 week excersise program
SA036183ms6169-411/25/2013 post-training Overweight/Obese adolescents 48 week excersise program
SA036184ms6169-2611/25/2014 post-training Overweight/Obese adolescents 48 week excersise program
SA036185ms6169-211/26/2013 post-training Overweight/Obese adolescents 48 week excersise program
SA036186ms6169-7811/28/2014 baseline Overweight/Obese adolescents 48 week excersise program
SA036160ms6169-961/15/2016 post-training Overweight/Obese adolescents 48 week excersise program
SA036187ms6169-5411/6/2015 post-training Overweight/Obese adolescents 48 week excersise program
SA036188ms6169-5611/6/2015 post-training Overweight/Obese adolescents 48 week excersise program
SA036161ms6169-751/20/2015 post-training Overweight/Obese adolescents 48 week excersise program
SA036189ms6169-8212/11/2014 baseline Overweight/Obese adolescents 48 week excersise program
SA036190ms6169-9412/11/2015 post-training Overweight/Obese adolescents 48 week excersise program
SA036191ms6169-2812/12/2014 baseline Overweight/Obese adolescents 48 week excersise program
SA036192ms6169-7312/16/2014 post-training Overweight/Obese adolescents 48 week excersise program
SA036193ms6169-10712/17/2015 baseline normal weight reference group adolescents
SA036194ms6169-10612/17/2015 baseline normal weight reference group adolescents
SA036195ms6169-6912/18/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036196ms6169-6812/18/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036197ms6169-612/23/2013 post-training Overweight/Obese adolescents 48 week excersise program
SA036198ms6169-2012/23/2014 baseline Overweight/Obese adolescents 48 week excersise program
SA036199ms6169-10812/23/2015 baseline normal weight reference group adolescents
SA036200ms6169-10012/29/2016 baseline Overweight/Obese adolescents 48 week excersise program
SA036201ms6169-9912/29/2016 baseline Overweight/Obese adolescents 48 week excersise program
SA036202ms6169-9212/29/2016 post-training Overweight/Obese adolescents 48 week excersise program
SA036203ms6169-8012/4/2014 baseline Overweight/Obese adolescents 48 week excersise program
SA036204ms6169-12612/8/2010 baseline Overweight/Obese adolescents 5 week exercise program
SA036205ms6169-9812/8/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036162ms6169-101/31/2014 post-training Overweight/Obese adolescents 48 week excersise program
SA036163ms6169-81/31/2014 post-training Overweight/Obese adolescents 48 week excersise program
SA036164ms6169-861/5/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036165ms6169-841/5/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036166ms6169-1011/5/2016 baseline Overweight/Obese adolescents 48 week excersise program
SA036167ms6169-1281/6/2011 baseline Overweight/Obese adolescents 5 week exercise program
SA036168ms6169-1091/9/2016 baseline normal weight reference group adolescents
SA036206ms6169-882/13/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036207ms6169-1292/14/2011 post-training Overweight/Obese adolescents 5 week exercise program
SA036208ms6169-1272/15/2011 post-training Overweight/Obese adolescents 5 week exercise program
SA036209ms6169-1302/18/2011 baseline Overweight/Obese adolescents 5 week exercise program
SA036210ms6169-302/20/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036211ms6169-322/20/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036212ms6169-172/21/2014 post-training Overweight/Obese adolescents 48 week excersise program
SA036213ms6169-122/7/2014 post-training Overweight/Obese adolescents 48 week excersise program
SA036214ms6169-712/8/2016 baseline Overweight/Obese adolescents 48 week excersise program
SA036215ms6169-702/8/2016 baseline Overweight/Obese adolescents 48 week excersise program
SA036216ms6169-622/9/2016 post-training Overweight/Obese adolescents 48 week excersise program
SA036217ms6169-642/9/2016 post-training Overweight/Obese adolescents 48 week excersise program
SA036218ms6169-363/13/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036219ms6169-343/13/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036220ms6169-223/14/2014 baseline Overweight/Obese adolescents 48 week excersise program
SA036221ms6169-443/16/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036222ms6169-463/16/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036223ms6169-143/17/2014 post-training Overweight/Obese adolescents 48 week excersise program
SA036224ms6169-193/17/2014 post-training Overweight/Obese adolescents 48 week excersise program
SA036225ms6169-423/27/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036226ms6169-383/27/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036227ms6169-403/27/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036228ms6169-773/4/2015 post-training Overweight/Obese adolescents 48 week excersise program
SA036229ms6169-673/4/2016 post-training Overweight/Obese adolescents 48 week excersise program
SA036230ms6169-814/10/2015 post-training Overweight/Obese adolescents 48 week excersise program
SA036231ms6169-1314/22/2011 post-training Overweight/Obese adolescents 5 week exercise program
SA036232ms6169-214/28/2014 post-training Overweight/Obese adolescents 48 week excersise program
SA036233ms6169-834/30/2015 post-training Overweight/Obese adolescents 48 week excersise program
SA036234ms6169-235/16/2014 baseline Overweight/Obese adolescents 48 week excersise program
SA036235ms6169-1325/20/2011 baseline Overweight/Obese adolescents 5 week exercise program
SA036236ms6169-495/22/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036237ms6169-1125/25/2010 baseline Overweight/Obese adolescents 5 week exercise program
SA036238ms6169-1385/26/2011 baseline Overweight/Obese adolescents 5 week exercise program
SA036239ms6169-1365/3/2011 baseline Overweight/Obese adolescents 5 week exercise program
SA036240ms6169-1345/3/2011 baseline Overweight/Obese adolescents 5 week exercise program
SA036241ms6169-485/5/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036242ms6169-875/8/2015 post-training Overweight/Obese adolescents 48 week excersise program
SA036243ms6169-295/8/2015 post-training Overweight/Obese adolescents 48 week excersise program
SA036244ms6169-855/8/2015 post-training Overweight/Obese adolescents 48 week excersise program
SA036245ms6169-795/8/2015 post-training Overweight/Obese adolescents 48 week excersise program
SA036247ms6169-516/11/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036246ms6169-1146/1/2010 baseline Overweight/Obese adolescents 5 week exercise program
SA036248ms6169-1166/2/2010 baseline Overweight/Obese adolescents 5 week exercise program
SA036249ms6169-1206/23/2010 baseline Overweight/Obese adolescents 5 week exercise program
SA036250ms6169-1186/23/2010 baseline Overweight/Obese adolescents 5 week exercise program
SA036251ms6169-1426/23/2011 baseline Overweight/Obese adolescents 5 week exercise program
SA036252ms6169-1136/25/2010 post-training Overweight/Obese adolescents 5 week exercise program
SA036253ms6169-576/30/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036254ms6169-596/30/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036255ms6169-1106/8/2010 baseline Overweight/Obese adolescents 5 week exercise program
SA036256ms6169-1376/8/2011 post-training Overweight/Obese adolescents 5 week exercise program
SA036257ms6169-1356/8/2011 post-training Overweight/Obese adolescents 5 week exercise program
SA036258ms6169-896/8/2015 baseline Overweight/Obese adolescents 48 week excersise program
SA036259ms6169-1527/1/2010 baseline Overweight/Obese adolescents 5 week exercise program
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Collection:

Collection ID:CO000658
Collection Summary:"Male and female American Indians 11-21 years old are being recruited from the Choctaw Nation Health Service Area of southeast Oklahoma. We use a collaborative approach as recently described by our American Indian Programs Coordinator (12). The primary characteristics of the three study groups are shown in Table 1. Group 1 is obese (Body mass index, BMI, ≥95th percentile for age- and sex-specific norms based on growth charts from the Centers for Disease Control), with pubertal maturation level ≥Tanner Stage 2, family history of T2D, and recent history of low physical activity. Low physical activity, defined as attaining <30 minutes of structured moderate-to-vigorous intensity exercise on 3 days/week over the preceding 3 months, is confirmed through questionnaires and use of an accelerometer. Groups 2 and 3 are both classified as normal weight (BMI greater than 20th and less than 85th percentile) with maturation level ≥Tanner Stage 2. Group 2 will have low physical activity (same as Group 1), while Group 3 will have high physical activity, defined as >30 minutes of structured moderate-to-vigorous intensity exercise on >3 days/week in the preceding 3 months. Additional eligibility criteria are: lack of diabetes or other potentially confounding metabolic disorders, able to safely exercise, willingness to complete the testing and participation schedule, and not on medications known to impact the stated outcomes. A medical history and physical exam is performed to assure suitability for the study during the initial screening."
Sample Type:Blood

Treatment:

Treatment ID:TR000678
Treatment Summary:Only Group 1 participants continue on to the exercise training program, which lasts 48 weeks. Follow-up measurements of the clinical and functional tests are performed at 16, 32, and 48 weeks; values at 16 weeks will be used for the current proposal to assure adequate sample size. Exercise sessions are performed at the Choctaw Wellness Centers, where fitness professionals provide instruction and supervision. Participants receive incentives for completing 20-60 minutes of moderate-to-vigorous physical activity (MVPA) on 3 days per week. MVPA is defined as exercise that elicits a heart rate ≥ 70% of peak heart rate. Several types of exercise can be selected, including walking, running, cycling, aerobic dance or other activities. To promote healthy behavior within the community and foster social support, all participants are invited to exercise with family members or friends if they choose. Exercise intensity is monitored by recording heart rate with a chest strap transmitter that outputs to a computer for quantification and data storage. Training sessions are also recorded in an exercise log by each participant and confirmed by fitness center staff.

Sample Preparation:

Sampleprep ID:SP000671
Sampleprep Summary:Part 1: Longer-term exercise training study: Samples #1-133. Overweight/Obese adolescents (IDs 434-001 through 434-132) enrolled into a 48-week exercise program (3 days per week). Samples submitted are from the pre-training (Baseline) and the end of the first 16 weeks (Post-training) times. Compliance with the exercise program varies widely among participants. An additional set of normal weight reference group adolescents (IDs 434-301 through 434-308) are included. Note that 15 OW/OB participants in the exercise group and all 8 NW reference group participants have only a single baseline sample. All samples were collected following overnight fast into EDTA (lavender top) glass tubes for preparation of plasma, kept on ice 20 minutes, spun, aliquoted and then kept frozen. None have been previously used. Part 2: Short-term exercise training study: Samples with ID 11xxx. Overweight/Obese adolescents completed a short-term exercise program, consisting of 10 sessions over 5 weeks. Samples were collected following overnight fast, before (Baseline) and after (Post-training) the training program. These samples are serum since plasma was used for other analyses. All tubes are marked with a unique ID number (shown in list below) in black marker. Additional sample details on the labels are listed in the accompanying Excel spreadsheet.

Combined analysis:

Analysis ID AN000973
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Acquity BEH C18 (150 x 2.1mm, 1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Quantum Ultra
Ion Mode POSITIVE
Units uM

Chromatography:

Chromatography ID:CH000698
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C18 (150 x 2.1mm, 1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000868
Analysis ID:AN000973
Instrument Name:Thermo Quantum Ultra
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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