Summary of Study ST000608

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000445. The data can be accessed directly via it's Project DOI: 10.21228/M8J60X This work is supported by NIH grant, U2C- DK119886.

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Study IDST000608
Study TitleComparing identified and statistically significant lipids and polar metabolites in 15-year old serum and dried blood spot samples for longitudinal studies
Study SummaryThe use of dried blood spots (DBS) has many advantages over traditional plasma and serum samples such as the smaller blood volume required, storage at room temperature, and ability to sample in remote locations. However, understanding the robustness of different analytes in DBS samples is essential, especially in older samples collected for longitudinal studies. Here we analyzed the stability of polar metabolites and lipids in DBS samples collected in 2000-2001 and stored at room temperature. The identified and statistically significant molecules were then compared to matched serum samples stored at –80°C to determine if the DBS samples could be effectively used in a longitudinal study following metabolic disease. A total of 400 polar metabolites and lipids were identified in the serum and DBS samples using gas chromatograph/mass spectrometry (GC/MS), liquid chromatography (LC)/MS, and LC/ion mobility spectrometry-MS (LC/IMS-MS). The identified polar metabolites overlapped well between the sample types, though only one statistically significant metabolite was conserved in a case-control study of older diabetic males with low amounts of high-density lipoproteins and high body mass indices, triacylglycerides and glucose levels when compared to non-diabetic patients with normal levels, indicating that degradation in the DBS samples affects polar metabolite quantitation. Differences in the lipid identifications indicated that some oxidation occurs in the DBS samples. However, 36 statistically significant lipids correlated in both sample types. The difference in the number of statistically significant polar metabolites and lipids indicated that the lipids did not degrade to as great of a degree as the polar metabolites in the DBS samples and lipid quantitation was still possible.
Institute
Pacific Northwest National Laboratory
Last NameBaker
First NameErin
Address902 Battelle Boulevard, Richland, WA, 99354, USA
Emailerin.baker@pnnl.gov
Phone509-371-6219
Submit Date2017-05-13
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailGC/LC-MS
Release Date2017-07-10
Release Version1
Erin Baker Erin Baker
https://dx.doi.org/10.21228/M8J60X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000929 AN000930 AN000931
Analysis type MS MS MS
Chromatography type Reversed phase Reversed phase GC
Chromatography system Waters NanoAcquity Waters NanoAcquity Agilent 7890A
Column Waters Acquity HSS T3 (150 x 1mm, 1.8um) Waters Acquity HSS T3 (150 x 1mm, 1.8um) Agilent HP5-MS (30m × 0.25mm, 0.25 um)
MS Type ESI ESI EI
MS instrument type Orbitrap Orbitrap Single quadrupole
MS instrument name Thermo Velos Pro Orbitrap Thermo Velos Pro Orbitrap Agilent 7890A
Ion Mode POSITIVE NEGATIVE POSITIVE
Units Peak Height Peak Peak Area
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