Summary of Study ST000608

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000445. The data can be accessed directly via it's Project DOI: 10.21228/M8J60X This work is supported by NIH grant, U2C- DK119886.

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Study IDST000608
Study TitleComparing identified and statistically significant lipids and polar metabolites in 15-year old serum and dried blood spot samples for longitudinal studies
Study SummaryThe use of dried blood spots (DBS) has many advantages over traditional plasma and serum samples such as the smaller blood volume required, storage at room temperature, and ability to sample in remote locations. However, understanding the robustness of different analytes in DBS samples is essential, especially in older samples collected for longitudinal studies. Here we analyzed the stability of polar metabolites and lipids in DBS samples collected in 2000-2001 and stored at room temperature. The identified and statistically significant molecules were then compared to matched serum samples stored at –80°C to determine if the DBS samples could be effectively used in a longitudinal study following metabolic disease. A total of 400 polar metabolites and lipids were identified in the serum and DBS samples using gas chromatograph/mass spectrometry (GC/MS), liquid chromatography (LC)/MS, and LC/ion mobility spectrometry-MS (LC/IMS-MS). The identified polar metabolites overlapped well between the sample types, though only one statistically significant metabolite was conserved in a case-control study of older diabetic males with low amounts of high-density lipoproteins and high body mass indices, triacylglycerides and glucose levels when compared to non-diabetic patients with normal levels, indicating that degradation in the DBS samples affects polar metabolite quantitation. Differences in the lipid identifications indicated that some oxidation occurs in the DBS samples. However, 36 statistically significant lipids correlated in both sample types. The difference in the number of statistically significant polar metabolites and lipids indicated that the lipids did not degrade to as great of a degree as the polar metabolites in the DBS samples and lipid quantitation was still possible.
Institute
Pacific Northwest National Laboratory
Last NameBaker
First NameErin
Address902 Battelle Boulevard, Richland, WA, 99354, USA
Emailerin.baker@pnnl.gov
Phone509-371-6219
Submit Date2017-05-13
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailGC/LC-MS
Release Date2017-07-10
Release Version1
Erin Baker Erin Baker
https://dx.doi.org/10.21228/M8J60X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample_Type Factor
SA033526OHSUblotter_PO6976_1blotter case
SA033527OHSUblotter_PO6927_3blotter case
SA033528OHSUblotter_PO6927_2blotter case
SA033529OHSUblotter_PO6976_2blotter case
SA033530OHSUblotter_PO6976_3blotter case
SA033531OHSUblotter_PO7324_3blotter case
SA033532OHSUblotter_PO7324_1blotter case
SA033533OHSUblotter_PO6927_1blotter case
SA033534OHSUblotter_PO7324_2blotter case
SA033535OHSUblotter_PO6716_2blotter case
SA033536OHSUblotter_PO6716_3blotter case
SA033537OHSUblotter_PO6764_2blotter case
SA033538OHSUblotter_PO6764_3blotter case
SA033539OHSUblotter_PO6764_1blotter case
SA033540OHSUblotter_PO6716_1blotter case
SA033541OHSUblotter_PO6573_3blotter control
SA033542OHSUblotter_PO7355_2blotter control
SA033543OHSUblotter_PO6573_2blotter control
SA033544OHSUblotter_PO6882_2blotter control
SA033545OHSUblotter_PO7355_3blotter control
SA033546OHSUblotter_PO7117_3blotter control
SA033547OHSUblotter_PO7355_1blotter control
SA033548OHSUblotter_PO6573_1blotter control
SA033549OHSUblotter_PO6935_1blotter control
SA033550OHSUblotter_PO6882_1blotter control
SA033551OHSUblotter_PO7117_2blotter control
SA033552OHSUblotter_PO6935_2blotter control
SA033553OHSUblotter_PO6882_3blotter control
SA033554OHSUblotter_PO6935_3blotter control
SA033555OHSUblotter_PO7117_1blotter control
SA033556OHSUserum_PO6976_3serum case
SA033557OHSUserum_PO6927_1serum case
SA033558OHSUserum_PO6927_3serum case
SA033559OHSUserum_PO6927_2serum case
SA033560OHSUserum_PO7324_1serum case
SA033561OHSUserum_PO7324_3serum case
SA033562OHSUserum_PO6976_2serum case
SA033563OHSUserum_PO6976_1serum case
SA033564OHSUserum_PO7324_2serum case
SA033565OHSUserum_PO6764_2serum case
SA033566OHSUserum_PO6716_2serum case
SA033567OHSUserum_PO6716_1serum case
SA033568OHSUserum_PO6764_1serum case
SA033569OHSUserum_PO6716_3serum case
SA033570OHSUserum_PO6764_3serum case
SA033571OHSUserum_PO7117_2serum control
SA033572OHSUserum_PO7355_3serum control
SA033573OHSUserum_PO7117_3serum control
SA033574OHSUserum_PO7355_1serum control
SA033575OHSUserum_PO7355_2serum control
SA033576OHSUserum_PO6573_1serum control
SA033577OHSUserum_PO6882_1serum control
SA033578OHSUserum_PO6573_3serum control
SA033579OHSUserum_PO6573_2serum control
SA033580OHSUserum_PO6882_2serum control
SA033581OHSUserum_PO6882_3serum control
SA033582OHSUserum_PO6935_3serum control
SA033583OHSUserum_PO6935_2serum control
SA033584OHSUserum_PO6935_1serum control
SA033585OHSUserum_PO7117_1serum control
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