Summary of Study ST001708

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001093. The data can be accessed directly via it's Project DOI: 10.21228/M8DT31 This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001708
Study TitleOxylipin biosynthesis reinforces cellular senescence through a RAS/p53 feedback loop and allows detection of senolysis
Study SummaryCellular senescence is a stress or damage response that causes a permanent proliferative arrest and secretion of numerous factors with potent biological activities. This senescence-associated secretory phenotype (SASP) has been characterized largely for secreted proteins that participate in embryogenesis, wound healing, inflammation and many age-related pathologies. By contrast, lipid components of the SASP are understudied. We show that senescent cells activate the biosynthesis of several oxylipins that promote segments of the SASP and reinforce the proliferative arrest. Notably, senescent cells synthesize and accumulate an unstudied intracellular prostaglandin, 1a,1b-dihomo-15-deoxy-delta-12,14-prostaglandin J2. Released 15-deoxy-delta-12,14-prostaglandin J2 is a biomarker of senolysis in culture and in vivo. This and other prostaglandin D2-related lipids promote the senescence arrest and SASP by activating RAS signaling. These data identify an important aspect of cellular senescence and a method to detect senolysis
Institute
Buck Institute for Research on Aging
Last NameSharma
First NameRishi
Address8001 Redwood Blvd, Novato, CA, 94945, USA
Emailsharmarishi2004@yahoo.co.in
Phone5084392367
Submit Date2021-02-20
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2021-08-10
Release Version1
Rishi Sharma Rishi Sharma
https://dx.doi.org/10.21228/M8DT31
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002782
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 6520
Column Phenomenex Kinetex C18 (150 x 2.1mm,2.6um)
MS Type ESI
MS instrument type QToF
MS instrument name Agilent 6520 QTOF
Ion Mode NEGATIVE
Units Peak Area
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