Summary of Study ST003114

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001935. The data can be accessed directly via it's Project DOI: 10.21228/M8M430 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003114
Study TitleLipidomics analyses in model membranes, isolated mitochondria and cellular systems to study how the local lipid environment affects BAX and BAK function during apoptosis.
Study SummaryTo investigate how the local lipid environment affects BAX and BAK function during apoptosis, we performed quantitative analyses of different lipid classes (glycerophospholipids, fatty acids, ceramides and sphingomyelins) in cultured cells, isolated mitochondria and lipid nanodics formed by Styrene-Malic Acid (SMA) co-polymers. Ceramides, sphingomyelins, fatty acids and cardiolipins were analyzed by Liquid Chromatography coupled to Tandem Mass Spectrometry (LC-MS/MS). For glycerophospholipids (PC, PE, PI, PS, PG, PA) we applied direct infusion MS approaches (Shotgun Lipidomics).
Institute
University of Cologne
DepartmentFaculty of Medicine and University Hospital of Cologne, Cluster of Excellence Cellular Stress Responses in Aging-associated Diseases (CECAD)
LaboratoryCECAD Lipidomics/Metabolomics Facility
Last NameBrodesser
First NameSusanne
AddressJoseph-Stelzmann-Str. 26, 50931 Cologne, Germany
Emailsusanne.brodesser@uk-koeln.de
Phone+49 221 478 84015
Submit Date2023-08-16
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-03-13
Release Version1
Susanne Brodesser Susanne Brodesser
https://dx.doi.org/10.21228/M8M430
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN005102 AN005103 AN005104 AN005105
Analysis type MS MS MS MS
Chromatography type None (Direct infusion) Normal phase Reversed phase Reversed phase
Chromatography system Advion TriVersa NanoMate Agilent 1260 Shimadzu Nexera X2 Shimadzu Nexera X2
Column None Macherey-Nagel Nucleosil NH2 (50×2 mm, 3 um, 120 Å) Waters Acquity BEH Shield RP18 (100×2.1 mm, 1.7 um) Phenomenex Core-Shell Kinetex Biphenyl (100×3.0 mm, 2.6 um, 100 Å)
MS Type ESI ESI ESI ESI
MS instrument type QTRAP QTRAP QTRAP QTRAP
MS instrument name SCIEX QTRAP 6500 SCIEX QTRAP 6500 SCIEX QTRAP 6500 SCIEX QTRAP 6500
Ion Mode POSITIVE POSITIVE POSITIVE NEGATIVE
Units counts per second (cps) counts per second (cps) counts per second (cps) counts per second (cps)
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