Summary of Study ST003114

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001935. The data can be accessed directly via it's Project DOI: 10.21228/M8M430 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003114
Study TitleLipidomics analyses in model membranes, isolated mitochondria and cellular systems to study how the local lipid environment affects BAX and BAK function during apoptosis.
Study SummaryTo investigate how the local lipid environment affects BAX and BAK function during apoptosis, we performed quantitative analyses of different lipid classes (glycerophospholipids, fatty acids, ceramides and sphingomyelins) in cultured cells, isolated mitochondria and lipid nanodics formed by Styrene-Malic Acid (SMA) co-polymers. Ceramides, sphingomyelins, fatty acids and cardiolipins were analyzed by Liquid Chromatography coupled to Tandem Mass Spectrometry (LC-MS/MS). For glycerophospholipids (PC, PE, PI, PS, PG, PA) we applied direct infusion MS approaches (Shotgun Lipidomics).
Institute
University of Cologne
DepartmentFaculty of Medicine and University Hospital of Cologne, Cluster of Excellence Cellular Stress Responses in Aging-associated Diseases (CECAD)
LaboratoryCECAD Lipidomics/Metabolomics Facility
Last NameBrodesser
First NameSusanne
AddressJoseph-Stelzmann-Str. 26, 50931 Cologne, Germany
Emailsusanne.brodesser@uk-koeln.de
Phone+49 221 478 84015
Submit Date2023-08-16
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2024-03-13
Release Version1
Susanne Brodesser Susanne Brodesser
https://dx.doi.org/10.21228/M8M430
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Cultured cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Genotype Condition
SA337372S07_pulldown_Apoptosis_01.09mitochondrial SMALPs mEGFP-BAK apoptotic
SA337373S09_pulldown_Apoptosis_31.09mitochondrial SMALPs mEGFP-BAK apoptotic
SA337374S08_pulldown_Apoptosis_04.09mitochondrial SMALPs mEGFP-BAK apoptotic
SA337375S06_pulldown_Apoptosis_22.08mitochondrial SMALPs mEGFP-BAK apoptotic
SA337376S03_pulldown_Control_01.09mitochondrial SMALPs mEGFP-BAK control
SA337377S04_pulldown_Control_04.09mitochondrial SMALPs mEGFP-BAK control
SA337378S02_pulldown_Control_22.08mitochondrial SMALPs mEGFP-BAK control
SA337379S05_pulldown_Control_31.09mitochondrial SMALPs mEGFP-BAK control
SA337364S07_mitos_apoptosis.SMA_3mitochondrial SMALPs WT apoptotic
SA337365S08_mitos_apoptosis.SMA_4mitochondrial SMALPs WT apoptotic
SA337366S05_mitos_apoptosis.SMA_1mitochondrial SMALPs WT apoptotic
SA337367S06_mitos_apoptosis.SMA_2mitochondrial SMALPs WT apoptotic
SA337368S01_mitos_control.SMA_1mitochondrial SMALPs WT control
SA337369S03_mitos_control.SMA_3mitochondrial SMALPs WT control
SA337370S02_mitos_control.SMA_2mitochondrial SMALPs WT control
SA337371S04_mitos_control.SMA_4mitochondrial SMALPs WT control
SA337380S14_mitos_linoleic.acid_KO_2total mitochondria FADS2 KO linoleic acid
SA337381S16_mitos_linoleic.acid_KO_4total mitochondria FADS2 KO linoleic acid
SA337382S15_mitos_linoleic.acid_KO_3total mitochondria FADS2 KO linoleic acid
SA337383S13_mitos_linoleic.acid_KO_1total mitochondria FADS2 KO linoleic acid
SA337384S10_mitos_no.treatment_KO_2total mitochondria FADS2 KO untreated
SA337385S09_smitos_no.treatment_KO_1total mitochondria FADS2 KO untreated
SA337386S11_mitos_no.treatment_KO_3total mitochondria FADS2 KO untreated
SA337387S12_mitos_no.treatment_KO_4total mitochondria FADS2 KO untreated
SA337388S14_mitos_apoptosis_2total mitochondria WT apoptotic
SA337389S13_mitos_apoptosis_1total mitochondria WT apoptotic
SA337390S15_mitos_apoptosis_3total mitochondria WT apoptotic
SA337391S16_mitos_apoptosis_4total mitochondria WT apoptotic
SA337392S09_mitos_control_1total mitochondria WT control
SA337393S11_mitos_control_3total mitochondria WT control
SA337394S12_mitos_control_4total mitochondria WT control
SA337395S10_mitos_control_2total mitochondria WT control
SA337396S05_mitos_linoleic.acid_WT_1total mitochondria WT linoleic acid
SA337397S08_mitos_linoleic.acid_WT_4total mitochondria WT linoleic acid
SA337398S06_mitos_linoleic.acid_WT_2total mitochondria WT linoleic acid
SA337399S07_mitos_linoleic.acid_WT_3total mitochondria WT linoleic acid
SA337400S03_mitos_no.treatment_WT_3total mitochondria WT untreated
SA337401S01_mitos_no.treatment_WT_1total mitochondria WT untreated
SA337402S02_mitos_no.treatment_WT_2total mitochondria WT untreated
SA337403S04_mitos_no.treatment_WT_4total mitochondria WT untreated
Showing results 1 to 40 of 40
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