Summary of Study ST000278

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000222. The data can be accessed directly via it's Project DOI: 10.21228/M8TP4R This work is supported by NIH grant, U2C- DK119886.


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Study IDST000278
Study TitleViral Effect on Metabolism (part I)
Study TypeGlycolysis/TCA/Nucleotide analysis (tissue/cells)
Study SummarySmall airway epithelial cell (SAEC) (Lonza Walkersville, Inc., MD) were grown according to the manufacturer's instructions in growth medium (SAGM) containing 0.03 mg/ml bovine pituitary extract (BPE), 0.5 µg/ml hydrocortisone, 0.5 ng/ml hEGF, 0.5 µg/ml epinephrine, 10 µg/ml transferrin, 5 µg/ml insulin, 0.1 ng/ml retinoic acid, 6.5ng/ml triiodothyronine, 50 µg/ml gentamicin and 50ng/ml Amphotericin-B, and 0.5 mg/ml bovine serum albumin (BSA, fatty acid free). When SAE grew to around 80 to 90% confluence, the cells were put into basal medium not supplemented with growth factors 3hours. Then the cell monolayers were infected with naïve hMPV at multiplicity of infection (MOI) of 3 to certain time. The supernatant was saved and cells were harvested and stored exactly according to the protocol provided by University of Michigan Metabolomics Core Facility for metabolomics analysis. 50mM ammonium acetate in water was used for rinse buffer.
University of Michigan
DepartmentDepartment of Pediatrics
LaboratoryCasola Lab (University of Texas Medical Branch)
Last NameZhao
First NameYanhua
AddressGalveston, TX
Submit Date2014-10-24
Num Groups4
Total Subjects12
Raw Data File Type(s)d
Analysis Type DetailGC-MS/LC-MS
Release Date2016-01-13
Release Version1
Yanhua Zhao Yanhua Zhao application/zip

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN000443 AN000444
Analysis type MS MS
Chromatography type HILIC GC
Chromatography system Agilent 1260 Agilent 7890A
Column Phenomenex Luna NH2 (150 x 1mm,3um) Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS instrument type QTOF Single quadrupole
MS instrument name Agilent 6530 QTOF Agilent 5975C
Units pmol/µg protein pmol/µg protein


Chromatography ID:CH000314
Methods ID:AQM020
Methods Filename:QTOF-002-HILIC-35min-1mm-No_Insert.m
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Chromatography Type:HILIC
Chromatography ID:CH001308
Methods ID:AQM011
Instrument Name:Agilent 7890A
Column Name:Agilent DB5-MS (30m × 0.25mm, 0.25um)
Chromatography Type:GC