Summary of Study ST000278

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000222. The data can be accessed directly via it's Project DOI: 10.21228/M8TP4R This work is supported by NIH grant, U2C- DK119886.


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Study IDST000278
Study TitleViral Effect on Metabolism (part I)
Study TypeGlycolysis/TCA/Nucleotide analysis (tissue/cells)
Study SummarySmall airway epithelial cell (SAEC) (Lonza Walkersville, Inc., MD) were grown according to the manufacturer's instructions in growth medium (SAGM) containing 0.03 mg/ml bovine pituitary extract (BPE), 0.5 µg/ml hydrocortisone, 0.5 ng/ml hEGF, 0.5 µg/ml epinephrine, 10 µg/ml transferrin, 5 µg/ml insulin, 0.1 ng/ml retinoic acid, 6.5ng/ml triiodothyronine, 50 µg/ml gentamicin and 50ng/ml Amphotericin-B, and 0.5 mg/ml bovine serum albumin (BSA, fatty acid free). When SAE grew to around 80 to 90% confluence, the cells were put into basal medium not supplemented with growth factors 3hours. Then the cell monolayers were infected with naïve hMPV at multiplicity of infection (MOI) of 3 to certain time. The supernatant was saved and cells were harvested and stored exactly according to the protocol provided by University of Michigan Metabolomics Core Facility for metabolomics analysis. 50mM ammonium acetate in water was used for rinse buffer.
University of Michigan
DepartmentDepartment of Pediatrics
LaboratoryCasola Lab (University of Texas Medical Branch)
Last NameZhao
First NameYanhua
AddressGalveston, TX
Submit Date2014-10-24
Num Groups4
Total Subjects12
Raw Data File Type(s)d
Analysis Type DetailGC-MS/LC-MS
Release Date2016-01-13
Release Version1
Yanhua Zhao Yanhua Zhao application/zip

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Treatment ID:TR000312