Summary of Study ST000351
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000280. The data can be accessed directly via it's Project DOI: 10.21228/M8QK55 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000351 |
Study Title | Determining the metabolic profile of wildtype, lrgAB, and atlA mutant Steptococcus mutans grown aerobically and anaerobically |
Study Type | Single time point, aerobic vs. anaerobic cultures |
Study Summary | Whole cells from both aerobic and anaerobic cultures of wild-type,lrgAB and atlA strain will be isolated for analysis of the whole cytosolic metabolome. Supernatants will be also analyzed for their metabolite profile in wild type and lrgAB cultures. |
Institute | University of Florida |
Department | SECIM |
Last Name | Ahn |
First Name | Sang-Joon |
Address | 1395 Center Drive, Room D5-29 Gainesville FL 32610 |
sahn@dental.ufl.edu | |
Phone | 352-273-8834 |
Submit Date | 2015-11-16 |
Num Groups | 3 |
Total Subjects | 36 |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2019-01-22 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000567 | AN000568 | AN000569 | AN000570 | AN000571 |
---|---|---|---|---|---|
Analysis type | MS | MS | MS | MS | MS |
Chromatography type | Reversed phase | Reversed phase | Reversed phase | Reversed phase | Reversed phase |
Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity | Thermo Dionex Ultimate 3000 | Agilent 1290 Infinity | Agilent 1290 Infinity |
Column | Waters AccQ Tag (100 x 2mm,1.7um) | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) | Waters Acquity BEH C18 (100 x 2mm,1.7um) | Thermo Hypercarb (50 x 2.1mm,3um) | Waters Acquity BEH C18 (50 x 2mm,1.7um) |
MS Type | ESI | ESI | ESI | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ | Agilent 6490 QQQ | Thermo Quantiva QQQ | Agilent 6490 QQQ | Agilent 6490 QQQ |
Ion Mode | POSITIVE | POSITIVE | POSITIVE | POSITIVE | POSITIVE |
Units | nmol/mg | nmol/mg | nmol/mg | nmol/mg | nmol/mg |
Chromatography:
Chromatography ID: | CH000404 |
Chromatography Summary: | Amino Acid method |
Methods Filename: | SB_AA_Assay.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters AccQ Tag (100 x 2mm,1.7um) |
Column Temperature: | 55 |
Flow Rate: | 0.7 mL/min |
Solvent A: | AccQ·Tag Eluent A |
Solvent B: | accQ·Tag Eluent B |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000405 |
Chromatography Summary: | Acylcarnitine method |
Methods Filename: | SB_Acylcarnitine_Assay.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters Acquity BEH C18 (100 x 2.1mm,1.7um) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000406 |
Chromatography Summary: | Organic acid method |
Methods Filename: | SB_OA_Assay.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | Waters Acquity BEH C18 (100 x 2mm,1.7um) |
Column Temperature: | 45 |
Flow Rate: | 0.3 mL/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000407 |
Chromatography Summary: | NAD-ATP method |
Methods Filename: | SB_NAD-ATP_Assay_Agilent.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Thermo Hypercarb (50 x 2.1mm,3um) |
Chromatography Type: | Reversed phase |
Chromatography ID: | CH000408 |
Chromatography Summary: | CoA method |
Methods Filename: | SB_CoA_Assay.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters Acquity BEH C18 (50 x 2mm,1.7um) |
Chromatography Type: | Reversed phase |