Summary of Study ST000351

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000280. The data can be accessed directly via it's Project DOI: 10.21228/M8QK55 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000351
Study TitleDetermining the metabolic profile of wildtype, lrgAB, and atlA mutant Steptococcus mutans grown aerobically and anaerobically
Study TypeSingle time point, aerobic vs. anaerobic cultures
Study SummaryWhole cells from both aerobic and anaerobic cultures of wild-type,lrgAB and atlA strain will be isolated for analysis of the whole cytosolic metabolome. Supernatants will be also analyzed for their metabolite profile in wild type and lrgAB cultures.
Institute
University of Florida
DepartmentSECIM
Last NameAhn
First NameSang-Joon
Address1395 Center Drive, Room D5-29 Gainesville FL 32610
Emailsahn@dental.ufl.edu
Phone352-273-8834
Submit Date2015-11-16
Num Groups3
Total Subjects36
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2019-01-22
Release Version1
Sang-Joon Ahn Sang-Joon Ahn
https://dx.doi.org/10.21228/M8QK55
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000567 AN000568 AN000569 AN000570 AN000571
Analysis type MS MS MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase Reversed phase Reversed phase
Chromatography system Agilent 1290 Infinity Agilent 1290 Infinity Thermo Dionex Ultimate 3000 Agilent 1290 Infinity Agilent 1290 Infinity
Column Waters AccQ Tag (100 x 2mm,1.7um) Waters Acquity BEH C18 (100 x 2.1mm,1.7um) Waters Acquity BEH C18 (100 x 2mm,1.7um) Thermo Hypercarb (50 x 2.1mm,3um) Waters Acquity BEH C18 (50 x 2mm,1.7um)
MS Type ESI ESI ESI ESI ESI
MS instrument type Triple quadrupole Triple quadrupole Triple quadrupole Triple quadrupole Triple quadrupole
MS instrument name Agilent 6490 QQQ Agilent 6490 QQQ Thermo Quantiva QQQ Agilent 6490 QQQ Agilent 6490 QQQ
Ion Mode POSITIVE POSITIVE POSITIVE POSITIVE POSITIVE
Units nmol/mg nmol/mg nmol/mg nmol/mg nmol/mg

Chromatography:

Chromatography ID:CH000404
Chromatography Summary:Amino Acid method
Methods Filename:SB_AA_Assay.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters AccQ Tag (100 x 2mm,1.7um)
Column Temperature:55
Flow Rate:0.7 mL/min
Solvent A:AccQ·Tag Eluent A
Solvent B:accQ·Tag Eluent B
Chromatography Type:Reversed phase
  
Chromatography ID:CH000405
Chromatography Summary:Acylcarnitine method
Methods Filename:SB_Acylcarnitine_Assay.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters Acquity BEH C18 (100 x 2.1mm,1.7um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH000406
Chromatography Summary:Organic acid method
Methods Filename:SB_OA_Assay.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Acquity BEH C18 (100 x 2mm,1.7um)
Column Temperature:45
Flow Rate:0.3 mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH000407
Chromatography Summary:NAD-ATP method
Methods Filename:SB_NAD-ATP_Assay_Agilent.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Thermo Hypercarb (50 x 2.1mm,3um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH000408
Chromatography Summary:CoA method
Methods Filename:SB_CoA_Assay.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters Acquity BEH C18 (50 x 2mm,1.7um)
Chromatography Type:Reversed phase
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