Summary of Study ST000351
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000280. The data can be accessed directly via it's Project DOI: 10.21228/M8QK55 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000351 |
| Study Title | Determining the metabolic profile of wildtype, lrgAB, and atlA mutant Steptococcus mutans grown aerobically and anaerobically |
| Study Type | Single time point, aerobic vs. anaerobic cultures |
| Study Summary | Whole cells from both aerobic and anaerobic cultures of wild-type,lrgAB and atlA strain will be isolated for analysis of the whole cytosolic metabolome. Supernatants will be also analyzed for their metabolite profile in wild type and lrgAB cultures. |
| Institute | University of Florida |
| Department | SECIM |
| Last Name | Ahn |
| First Name | Sang-Joon |
| Address | 1395 Center Drive, Room D5-29 Gainesville FL 32610 |
| sahn@dental.ufl.edu | |
| Phone | 352-273-8834 |
| Submit Date | 2015-11-16 |
| Num Groups | 3 |
| Total Subjects | 36 |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-01-22 |
| Release Version | 1 |
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Project:
| Project ID: | PR000280 |
| Project DOI: | doi: 10.21228/M8QK55 |
| Project Title: | Application of a targeted metabolomic profiling for the delineation of mechanisms of autolytic systems in Streptococcus mutans cells under oxidative stresses |
| Project Summary: | Streptococcus mutans is capable of withstanding a variety of stressors encountered in the oral cavity. The ability to efficiently and rapidly adjust to the changing environment is essential for its pathogenic lifestyle. Oxidative stress is one of the most important environmental variables affecting the pathogenic potential of S. mutans, as high oxygen concentrations disfavor growth of S. mutans and other oral bacteria. In order to fulfill its role as a major constituent of cariogenic oral biofilms and sustain virulence, S. mutans must overcome oxidative stress. Oxidation sensing and response is a complex yet highly-regulated process, particularly because it is cross-regulated with other metabolic pathways, stress responses and virulence physiology. In addition, we previously showed that exposure of S. mutans to oxygen strongly inhibits biofilm formation and alters cell surface biogenesis, possibly through AtlA, a major autolysin of the organism. More recently, we have also shown that S. mutans Cid/Lrg system, potentially mediating cell lysis/death in a programmed fashion, uniquely leads a global readjustment in central metabolism and virulence processes, particularly in coping with oxidative stress. To this end, a targeted metabolomics approach will be employed to understand how these autolytic systems influence the metabolism of S. mutans, ultimately leading to a more complete appreciation of how S. mutans survives and persists in hostile host microenvironment. |
| Institute: | University of Florida |
| Department: | College of Dentistry |
| Last Name: | Ahn |
| First Name: | Sang-Joon |
| Address: | 1395 Center Drive, Room D5-29 Gainesville FL 32610 |
| Email: | sahn@dental.ufl.edu |
| Phone: | 352-273-8834 |
| Funding Source: | NIH R03 |
