Summary of Study ST000496
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000373. The data can be accessed directly via it's Project DOI: 10.21228/M8TW22 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000496 |
| Study Title | Distinct signatures of dental plaque metabolic byproducts dictated by periodontal inflammatory status |
| Study Summary | Onset of chronic periodontitis is associated with an aberrant polymicrobial community, termed dysbiosis. Findings of a recent model of its etiology suggested that dysbiosis holds a conserved metabolic signature as an emergent property. The purpose of this study was to identify robust biomarkers for periodontal inflammation severity. Furthermore, we investigated disease-associated metabolic signatures of periodontal microbiota using a salivary metabolomics approach. Collection of whole saliva samples was performed before and after removal of supragingival plaque (debridement). Periodontal inflamed surface area (PISA) was employed as an indicator of periodontal inflammatory status. Based on multivariate analyses using pre-debridement salivary metabolomics data, we found that the metabolites associated with higher PISA included cadaverine and hydrocinnamate, while uric acid and ethanolamine were associated with lower PISA. Next, we focused on dental plaque metabolic byproducts by selecting significantly decreased salivary metabolites following debridement. Metabolite set enrichment analysis revealed that polyamine metabolism, arginine and proline metabolism, butyric acid metabolism, and lysine degradation were distinctive metabolic signatures of dental plaque in the high PISA group, which may have relevance to the metabolic signatures of disease-associated communities. Collectively, our findings identified potential biomarkers of periodontal inflammatory status, while they also provide insight into metabolic signatures of dysbiotic communities. |
| Institute | Osaka University |
| Department | Graduate School of Dentistry |
| Last Name | Kuboniwa |
| First Name | Masae |
| Address | 1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan |
| kuboniwa@dent.osaka-u.ac.jp | |
| Phone | +81-6-6879-2922 |
| Submit Date | 2016-10-23 |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2017-11-20 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000762 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Shimadzu GCMS-QP2010 ultra |
| Column | a 30 m × 0.25 mm i.d. fused silica capillary column coated with 0.25 µm CP-SIL 8 CB low bleed (Varian Inc., Palo Alto, CA) |
| MS Type | EI |
| MS instrument type | GC-TOF |
| MS instrument name | Shimadzu QP2010 Ultra |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH000548 |
| Chromatography Summary: | The device used was GCMS-QP2010 ultra (Shimadzu Inc., Kyoto, Japan) equipped with a 30 m × 0.25 mm i.d. fused silica capillary column coated with 0.25 µm CP-SIL 8 CB low bleed (Varian Inc., Palo Alto, CA) and AOC-20i (Shimadzu) as an autosampler. The injection temperature was 230 °C. The helium gas flow rate through the column was 1.12 ml/min. The column temperature was held at 80 °C for 2 min and then raised by 15 °C /min to 330 °C and held for 6 min. The transfer line and the ion source temperatures were 250 °C and 200 °C, respectively. Ions were generated by a 70 kV electron impact (EI), and 20 scans/sec were recorded over the mass range 85-500. A standard alkane mixture (C8-C40) was injected at the beginning of the analysis for subsequent identification of metabolites. |
| Instrument Name: | Shimadzu GCMS-QP2010 ultra |
| Column Name: | a 30 m × 0.25 mm i.d. fused silica capillary column coated with 0.25 µm CP-SIL 8 CB low bleed (Varian Inc., Palo Alto, CA) |
| Column Temperature: | The column temperature was held at 80 °C for 2 min and then raised by 15 °C /min to 330 °C and held for 6 min. |
| Flow Rate: | The helium gas flow rate through the column was 1.12 ml/min. |
| Sample Injection: | 1 µL |
| Chromatography Type: | GC |
