Summary of Study ST001394

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000957. The data can be accessed directly via it's Project DOI: 10.21228/M80D7P This work is supported by NIH grant, U2C- DK119886.


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Study IDST001394
Study TitleMetabolomic study of Escherichia coli K-12 MG1655 and mutants
Study TypeMetabolomics
Study SummaryMetabolomic analysis of Wildtype, crp mutant and its five adaptively evolved populations evolved in glucose minimal media with 40 mM MOPS during its exponential phase of growth. Three biological and two technical replicate samples (n=6) were harvested for each of the strains while growing in a bioreactor aerobically at 37 degree Celsius and 700 rpm. This study aims to characterize and compare the metabolic profile of all these strains.
IIT Bombay
DepartmentDepartment of Chemical Engineering
LaboratorySystems Biology and Metabolic Engineering Laboratory
Last NamePal
First NameAnkita
AddressIIT Bombay, Powai, Mumbai - 400076, Maharashtra, India
Submit Date2020-06-06
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-03-01
Release Version1
Ankita Pal Ankita Pal application/zip

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Combined analysis:

Analysis ID AN002332 AN002333
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column SeQuant ZIC-HILIC (150 x 2.1mm,5um) SeQuant ZIC-HILIC (150 x 2.1mm,5um)
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap
Units Height ratio/gDCW Height ratio/gDCW


Chromatography ID:CH001709
Chromatography Summary:The detailed chromatography and MS protocol has been described in "ms_method.pdf" file.
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:SeQuant ZIC-HILIC (150 x 2.1mm,5um)
Chromatography Type:HILIC