Summary of Study ST001774
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001128. The data can be accessed directly via it's Project DOI: 10.21228/M8WQ4Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001774 |
| Study Title | Timecourse on primary human highly differentiated airway epithelial cells infected with HRV-C15 |
| Study Summary | At each time point (4, 12, 24 h), ALI basolateral media was collected and incubated 1:1 in ice-cold 100% methanol for 30 minutes on ice, vortexing every 10 minutes. Basolateral media was centrifuged at 20,000 × 𝑔 for 10 minutes at 4°C, and further diluted in 50% methanol prior to mass spectrometry plate loading. |
| Institute | McGill University |
| Last Name | Lopes |
| First Name | Fernando |
| Address | 21111 Lakeshore Rd |
| fernando.lopes@mcgill.ca | |
| Phone | 5143987607 |
| Submit Date | 2021-05-03 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-05-12 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002881 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Thermo Vanquish |
| Column | ThermoFisher Syncronis HILIC UHPLC (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Single quadrupole |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | NEGATIVE |
| Units | pmoles/l |
Chromatography:
| Chromatography ID: | CH002136 |
| Chromatography Summary: | General metabolomic run were performed on a Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ Mass Spectrometer (ThermoFisher) coupled to a Vanquish™ UHPLC System (ThermoFisher). Chromatographic separation was achieved on a Syncronis HILIC UHPLC column (2.1 mm x 100 mm x 1.7 mm, ThermoFisher) using a binary solvent system at a flow rate of 600 mL/min. Solvent A, 20 mM ammonium formate pH 3.0 in mass spectrometry grade H2O; Solvent B, mass spectrometry grade acetonitrile with 0.1% formic acid (% v/v). A sample injection volume of 2 mL was used. |
| Instrument Name: | Thermo Vanquish |
| Column Name: | ThermoFisher Syncronis HILIC UHPLC (100 x 2.1mm,1.7um) |
| Solvent A: | 100% water; 20 mM ammonium formate, pH 3 |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | HILIC |
