Summary of Study ST001774

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001128. The data can be accessed directly via it's Project DOI: 10.21228/M8WQ4Q This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001774
Study TitleTimecourse on primary human highly differentiated airway epithelial cells infected with HRV-C15
Study SummaryAt each time point (4, 12, 24 h), ALI basolateral media was collected and incubated 1:1 in ice-cold 100% methanol for 30 minutes on ice, vortexing every 10 minutes. Basolateral media was centrifuged at 20,000 × 𝑔 for 10 minutes at 4°C, and further diluted in 50% methanol prior to mass spectrometry plate loading.
Institute
McGill University
Last NameLopes
First NameFernando
Address21111 Lakeshore Rd
Emailfernando.lopes@mcgill.ca
Phone5143987607
Submit Date2021-05-03
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2021-05-12
Release Version1
Fernando Lopes Fernando Lopes
https://dx.doi.org/10.21228/M8WQ4Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002881
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column ThermoFisher Syncronis HILIC UHPLC (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Single quadrupole
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Ion Mode NEGATIVE
Units pmoles/l

MS:

MS ID:MS002674
Analysis ID:AN002881
Instrument Name:Thermo Q Exactive HF hybrid Orbitrap
Instrument Type:Single quadrupole
MS Type:ESI
MS Comments:The mass spectrometer was run in negative full scan mode at a resolution of 240,000 scanning from 50-750 m/z.
Ion Mode:NEGATIVE
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