Summary of Study ST001780

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001133. The data can be accessed directly via it's Project DOI: 10.21228/M87X24 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001780
Study TitleComparative analysis of metabolomic profiles in cerebrospinal fluid before and after endurance exercise
Study SummaryThis study compared the metabolomic differences in the cerebrospinal fluid of young adult volunteers between before (Pre), and 60 minutes after the 90-min run (Post).
Institute
University of California, San Diego School of Medicine
Last NameNaviaux
First NameRobert
Address214 Dickinson St., Room C-107, San Diego, CA, 92103, USA
Emailrnaviaux@health.ucsd.edu
Phone619-543-2904
Submit Date2021-05-03
Num Groups2
Total Subjects19
Num Males13
Num Females6
Analysis Type DetailLC-MS
Release Date2021-11-25
Release Version1
Robert Naviaux Robert Naviaux
https://dx.doi.org/10.21228/M87X24
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002890 AN002891
Analysis type MS MS
Chromatography type HILIC Reversed phase
Chromatography system Shimadzu 20AD Shimadzu 20AD
Column Shodex NH2P-40 2E (250 x 2mm,4um) Raptor Biphenyl (150 × 2.1 mm,2.7um)
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap ABI Sciex 5500 QTrap
Ion Mode UNSPECIFIED UNSPECIFIED
Units peak area peak area

Chromatography:

Chromatography ID:CH002142
Chromatography Summary:Ten µL of the extract was injected into the column through a CTC PAL autosampler. The samples were separated in HILIC mode using a polymer-based NH2 column (250 × 2 mm, 4 µm) (Asahipak NH2P-40 2E, Shodex, Japan). The optimal LC conditions were as follows: Mobile phase A: 95% H2O with 20 mM (NH4)2CO3 and 5% ACN, pH 9.8. Mobile phase B: 100% ACN. The gradient was: 0 - 3.5 min 95% B, 3.6 - 8 min 85% B, 8.1 - 13 min 75% B, 14 - 30 min 0% B, 31 - 41 min 95% B, 41.1 min stop. The flow rate was 200 µL/min, and the column temperature was held at 25 ºC.
Instrument Name:Shimadzu 20AD
Column Name:Shodex NH2P-40 2E (250 x 2mm,4um)
Column Pressure:900 psi - 2400 psi
Column Temperature:25
Flow Gradient:Constant
Flow Rate:200 µL/min
Injection Temperature:4
Sample Injection:10 uL
Solvent A:95% water/5% acetonitrile; 20 mM ammonium acetate, pH 9.8
Solvent B:100% acetonitrile
Analytical Time:45 min
Oven Temperature:25
Sample Loop Size:10
Sample Syringe Size:100
Chromatography Type:HILIC
  
Chromatography ID:CH002143
Chromatography Summary:Ten µL of the extract was injected into the column through a CTC PAL autosampler. The samples were separated in the RP model using a Raptor Biphenyl column (150 × 2.1 mm, 2.7 µm) (Restek, PA). The LC conditions were as follows: Mobile phase A: 90% H2O with 0.1% formic acid and 10% MEOH, pH 4.0. Mobile phase B: MEOH-IPA (50:50, v/v) with 0.1% formic acid. The gradient was: 0 - 2 min 10% B, 2.1 - 4 min 40% B, 4 -12 min, linear ramping up to 100% B, 12 – 18 min 100% B, 19 - 24 min 10% B, 24.1 min stop. The flow rate was 250 µL/min, and the column temperature was controlled at 40 ºC.
Instrument Name:Shimadzu 20AD
Column Name:Raptor Biphenyl (150 × 2.1 mm,2.7um)
Column Pressure:2800 psi - 4200 psi
Column Temperature:40
Flow Gradient:Constant
Flow Rate:250 µL/min
Injection Temperature:4
Sample Injection:10 uL
Solvent A:90% water/10% methanol; 0.1% formic acid, pH 4.0
Solvent B:50% methanol/50% isopropanol; 0.1% formic acid
Analytical Time:25 min
Oven Temperature:40
Sample Loop Size:10
Sample Syringe Size:100
Chromatography Type:Reversed phase
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