Summary of Study ST001822

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001151. The data can be accessed directly via it's Project DOI: 10.21228/M8XH6Q This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001822
Study TitleThe RNA-binding protein RBP42 regulates cellular energy metabolism in mammalian-infective Trypanosoma brucei
Study SummaryMetabolic changes following two days of RBP42 knockdown was investigated using a targeted metabolomics approach, designed to capture intermediary metabolites in central carbon metabolism including glycolytic intermediates, TCA compounds, amino acids, nucleotides and derivatives, were obtained using hydrophilic interaction liquid chromatography (HILIC) separation method coupled with mass spectrometry run in negative ionization mode
Institute
Rutgers University
Last NameDas
First NameAnish
AddressDepartment of Microbiology, Biochemistry and Molecular Genetics, Rutgers-New Jersey Medical School, Newark, NJ 07103
Emaildasak@njms.rutgers.edu
Phone9739723978
Submit Date2021-06-07
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2021-06-10
Release Version1
Anish Das Anish Das
https://dx.doi.org/10.21228/M8XH6Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002958
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap
Ion Mode NEGATIVE
Units arbitrary

Chromatography:

Chromatography ID:CH002191
Chromatography Summary:HILIC separation was performed on a Vanquish Horizon UHPLC system (Thermo Fisher Scientific, Waltham, MA) with an XBridge BEH Amide column (150 mm × 2.1 mm, 2.5 μm particle size, Waters, Milford, MA) using a gradient of solvent A (95%:5% H2O:acetonitrile with 20 mM acetic acid, 40 mM ammonium hydroxide, pH 9.4) and solvent B (20%:80% H2O:acetonitrile with 20 mM acetic acid, 40 mM ammonium hydroxide, pH 9.4). The gradient was 0 min, 100% B; 3 min, 100% B; 3.2 min, 90% B; 6.2 min, 90% B; 6.5 min, 80% B; 10.5 min, 80% B; 10.7 min, 70% B; 13.5 min, 70% B; 13.7 min, 45% B; 16 min, 45% B; 16.5 min, 100% B; and 22 min, 100% B. The flow rate was 300 μl/min. The injection volume was 5 μL, and the column temperature was set to 25°C. The autosampler temperature was set to 4°C, and the injection volume was 5 µL.
Instrument Name:Thermo Vanquish
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Column Temperature:25
Flow Gradient:0 min, 100% B; 3 min, 100% B; 3.2 min, 90% B; 6.2 min, 90% B; 6.5 min, 80% B; 10.5 min, 80% B; 10.7 min, 70% B; 13.5 min, 70% B; 13.7 min, 45% B; 16 min, 45% B; 16.5 min, 100% B; and 22 min, 100% B.
Flow Rate:300 µl/min
Solvent A:95% water/5% acetonitrile; 20 mM acetic acid; 40 mM ammonium hydroxide, pH 9.4
Solvent B:20% water/80% acetonitrile; 20 mM acetic acid, 40 mM ammonium hydroxide, pH 9.4
Chromatography Type:HILIC
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