Summary of Study ST001822

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001151. The data can be accessed directly via it's Project DOI: 10.21228/M8XH6Q This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001822
Study TitleThe RNA-binding protein RBP42 regulates cellular energy metabolism in mammalian-infective Trypanosoma brucei
Study SummaryMetabolic changes following two days of RBP42 knockdown was investigated using a targeted metabolomics approach, designed to capture intermediary metabolites in central carbon metabolism including glycolytic intermediates, TCA compounds, amino acids, nucleotides and derivatives, were obtained using hydrophilic interaction liquid chromatography (HILIC) separation method coupled with mass spectrometry run in negative ionization mode
Institute
Rutgers University
Last NameDas
First NameAnish
AddressDepartment of Microbiology, Biochemistry and Molecular Genetics, Rutgers-New Jersey Medical School, Newark, NJ 07103
Emaildasak@njms.rutgers.edu
Phone9739723978
Submit Date2021-06-07
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2021-06-10
Release Version1
Anish Das Anish Das
https://dx.doi.org/10.21228/M8XH6Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001905
Sampleprep Summary:Metabolites were extracted with 1 ml of 40:40:20 mixture of methanol:acetonitrile:water plus 0.5% (V/V) formic acid on ice for 5 min. Following neutralization of formic acid by addition of 50 ul of 15% (m/V) NH4HCO3, cleared extracts were collected by centrifugation at 15000g for 10 min and stored at -80C.
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