Summary of Study ST002066

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001308. The data can be accessed directly via it's Project DOI: 10.21228/M8N98X This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST002066
Study TitleGlutaminase inhibition impairs CD8 T cell activation in STK11/Lkb1 deficient lung cancer
Study TypeBiomedical research
Study SummaryTo characterize the impact of KRAS co-mutations KEAP1 and STK11/Lkb1 on the metabolic and immune microenvironment of lung adenocarcinoma in immune-intact models, we generated a cohort of genetically engineered mouse models (GEMMs) to reflect the diverse tumor suppressor landscape seen in patients. Mice carrying the KrasG12D allele (K)21 were crossed with Keap1flox/flox (KK)22 and/or Lkb1flox/flox (KKL, KL)23 mice and genetic recombination induced by intranasal inhalation of Ad5-CMV-Cre adenovirus. We interrogated the metabolites present in lung tumor nodules collected from cohorts of KK, KL and KKL mice.
Institute
The Walter and Eliza Hall Institute of Medical Research
LaboratoryKate Sutherland
Last NameSarah
First NameBest
Address1G, Royal Parade, Parkville VIC 3052, Australia
Emailbest@wehi.edu.au
Phone+61-3-9345-2452
Submit Date2022-01-18
Num Groups5
Total Subjects25
Num Males17
Num Females8
Study CommentsL lobe of mice lung
PublicationsGlutaminase inhibition impairs CD8 T cell activation in STK11/Lkb1 deficient lung cancer
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-05-02
Release Version1
Best Sarah Best Sarah
https://dx.doi.org/10.21228/M8N98X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Combined analysis:

Analysis ID AN003365 AN003366
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS
Column SeQuant ZIC-pHILIC (150 x 4.2 mm, 5 um) SeQuant ZIC-pHILIC (150 x 4.2 mm, 5 um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units

Chromatography:

Chromatography ID:CH002489
Chromatography Summary:LCMS data was acquired on Q-Exactive Orbitrap mass spectrometer (Thermo Fisher) coupled with high-performance liquid chromatography (HPLC) system Dionex Ultimate® 3000 RS (Thermo Fisher). Chromatographic separation was performed on a ZIC-pHILIC column (5 µm, polymeric, 150 × 4.6 mm, SeQuant®, Merck). The mobile phase (A) was 20 mM ammonium carbonate and (B) acetonitrile. The gradient program started at 80% B and was reduced to 50% B over 15 min, then reduced from 50% B to 5% B over 3 min, followed by wash with 5% B for another 3 min, and finally 8 min re-equilibration with 80% B. The flow rate was 0.3 mL/min and column compartment temperature was 40ºC. The total run time was 32 min with an injection sample volume of 10 µL.
Methods Filename:Metabolomics_pHILIC_Parkville_v1.meth
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:SeQuant ZIC-pHILIC (150 x 4.2 mm, 5 um)
Column Pressure:60 bar at starting conditions
Column Temperature:25 C
Flow Gradient:0 min - 80%B, 15 min - 50%B, 18 min - 5%B, 21 min - 5%B, 24 min - 80%B, 32 min - 80%B
Flow Rate:0.3 ml/min
Injection Temperature:4 C
Internal Standard:CAPS, CHAPS, PIPES
Sample Injection:10 uL
Solvent A:20 mM ammonium formate
Solvent B:acetonitrile
Analytical Time:32 min
Capillary Voltage:3.5 kV
Oven Temperature:25 C
Washing Buffer:syringe wash 50% IPA
Weak Wash Solvent Name:10% MeOH
Strong Wash Solvent Name:10% MeOH
Sample Loop Size:25 uL
Sample Syringe Size:25 uL
Chromatography Type:HILIC
  logo