Summary of Study ST002115
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001340. The data can be accessed directly via it's Project DOI: 10.21228/M8HD8Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002115 |
| Study Title | LC-MS analysis of metabolic changes induced by GPX4 inhibitor treatment in cultured HT1080 cells |
| Study Summary | HT1080 cells were treated with vehicle (DMSO), RSL3 (10 micromolar), ML210 (10 micromolar), or ML162 (10 micromolar) for 2 hours. Cellular metabolites were then extracted and analyzed by LC-MS. |
| Institute | University of Texas MD Anderson Cancer Center |
| Last Name | Gan |
| First Name | Boyi |
| Address | 6565 MD Anderson Blvd, Houston TX, 77030 |
| bgan@mdanderson.org | |
| Phone | 713-792-8653 |
| Submit Date | 2022-03-02 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2022-04-14 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003513 | AN003514 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | HILIC |
| Chromatography system | Thermo Accela 1250 | Thermo Accela 1250 |
| Column | Phenomenex Synergi Hydro RP 100 A (100 x 2mm,2.5um) | Waters XBridge BEH Amide (150 x 2.1mm,2.5um,100A) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
| Ion Mode | NEGATIVE | NEGATIVE |
| Units | Peak area (top) | Peak area (top) |
Chromatography:
| Chromatography ID: | CH002593 |
| Chromatography Summary: | The gradient was 0 min, 0% B; 2.5 min, 0% B; 5 min, 20% B; 7.5 min, 20% B; 13 min, 55% B; 15.5 min, 95% B; 18.5 min, 95% B; 19 min, 0% B; and 25 min, 0% B. Solvent A was 10 mM tributylamine and 15 mm acetic acid in water; Solvent B was methanol. The injection volume was 10 µL. |
| Instrument Name: | Thermo Accela 1250 |
| Column Name: | Phenomenex Synergi Hydro RP 100 A (100 x 2mm,2.5um) |
| Column Temperature: | 40 |
| Flow Rate: | 200 |
| Solvent A: | 100% water; 15 mM acetic acid; 10 mM tributylamine |
| Solvent B: | 100% methanol |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH002594 |
| Chromatography Summary: | The gradient was 0 min, 85% B; 2 min, 85% B; 3 min, 80% B; 5 min, 80% B; 6 min, 75% B; 7 min, 75% B; 8 min, 70% B; 9 min, 70% B; 10 min, 50% B; 12 min, 50% B; 13 min, 25% B; 16 min, 25% B; 18 min, 0% B; 23 min, 0% B; 24 min, 85% B; 30 min, 85% B. The injection volume was 5 µL. |
| Instrument Name: | Thermo Accela 1250 |
| Column Name: | Waters XBridge BEH Amide (150 x 2.1mm,2.5um,100A) |
| Column Temperature: | 40 |
| Flow Rate: | 150 |
| Solvent A: | 95% water/5% acetonitrile; 20 mM ammonium acetate, pH 9.4 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
