Summary of Study ST002115

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001340. The data can be accessed directly via it's Project DOI: 10.21228/M8HD8Q This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002115
Study TitleLC-MS analysis of metabolic changes induced by GPX4 inhibitor treatment in cultured HT1080 cells
Study SummaryHT1080 cells were treated with vehicle (DMSO), RSL3 (10 micromolar), ML210 (10 micromolar), or ML162 (10 micromolar) for 2 hours. Cellular metabolites were then extracted and analyzed by LC-MS.
Institute
University of Texas MD Anderson Cancer Center
Last NameGan
First NameBoyi
Address6565 MD Anderson Blvd, Houston TX, 77030
Emailbgan@mdanderson.org
Phone713-792-8653
Submit Date2022-03-02
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2022-04-14
Release Version1
Boyi Gan Boyi Gan
https://dx.doi.org/10.21228/M8HD8Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO002224
Collection Summary:Metabolites were extracted from cells in 35 mm culture plates by rapidly aspirating the culture medium and incubating the plates with 0.6 ml of an 80% methanol: 20% water mixture on a cold block on dry ice for 15 min. Next, the cell material was scraped into Eppendorf tubes pre-chilled on ice. After centrifugation at 13,000 RCF for 5 min at 4 °C, the supernatant was collected into a fresh tube and stored on dry ice until analysis.
Sample Type:Cultured cells
Storage Conditions:-80?
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