Summary of Study ST002289

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001468. The data can be accessed directly via it's Project DOI: 10.21228/M8ZT5R This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002289
Study TitleMap of microbially induced metabolic changes across diverse body sites in mice - Bacterial culture data
Study SummaryMetabolite profiles of culture supernatants from individual commensal bacteria isolated from the intestine of mice.
Institute
University of Calgary
DepartmentPhysiology and Pharmacology
LaboratoryMcCoy
Last NameBROWN
First NameKIRSTY
Address3330 Hospital Dr NW
Emailkirsty.brown1@ucalgary.ca
Phone2508692232
Submit Date2022-05-26
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2023-02-05
Release Version1
KIRSTY BROWN KIRSTY BROWN
https://dx.doi.org/10.21228/M8ZT5R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003741
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column Thermo Syncronis HILIC (100 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap
Ion Mode NEGATIVE
Units AUC

Chromatography:

Chromatography ID:CH002772
Methods Filename:culture_protocol_kb.docx
Instrument Name:Thermo Vanquish
Column Name:Thermo Syncronis HILIC (100 x 2.1mm,1.7um)
Column Temperature:30C
Flow Gradient:2 stage linear gradient
Flow Rate:600uL/min
Sample Injection:2uL
Solvent A:100% water; 20 mM ammonium formate, pH 3
Solvent B:100% acetonitrile; 0.1% formic acid
Analytical Time:15 minutes
Washing Buffer:10% MeOH in H2O + 0.1% formic acid
Target Sample Temperature:4
Chromatography Type:HILIC
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