Summary of Study ST002289
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001468. The data can be accessed directly via it's Project DOI: 10.21228/M8ZT5R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002289 |
Study Title | Map of microbially induced metabolic changes across diverse body sites in mice - Bacterial culture data |
Study Summary | Metabolite profiles of culture supernatants from individual commensal bacteria isolated from the intestine of mice. |
Institute | University of Calgary |
Department | Physiology and Pharmacology |
Laboratory | McCoy |
Last Name | BROWN |
First Name | KIRSTY |
Address | 3330 Hospital Dr NW |
kirsty.brown1@ucalgary.ca | |
Phone | 2508692232 |
Submit Date | 2022-05-26 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2023-02-05 |
Release Version | 1 |
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Collection:
Collection ID: | CO002368 |
Collection Summary: | Bacteria were grown in 15mL culture tubes in 3mL of modified brain heart infusion broth [37g/L BHI powder, 0.025% Cystiene-HCl.H2O, 0.025% Na2S.9H2O, 1ug/mL Hemin, 0.5ug/mL menadione, 0.025% mucin]. Media was pre-reduced in an anaerobic chamber (Whitley A95 Workstation; 90% N2, 5% H2 & 5% CO2) for 48 hours then bacterial cultures were started from glycerol stocks. 200µL of glycerol stock was added to each culture tube and cultures were incubated for 6-36 hours until early plateau phase. After incubation, cultures were removed from the anaerobic chamber, and 50uL was collected to extract DNA for 16S sequencing to confirm accurate identification of bacteria and absence of contamination. The remaining culture was centrifuged (max. speed, 15 minutes, 4°C) and the supernatant was collected and combined 1:1 with 100% methanol. Samples were then incubated at - 20°C for 1 hour. Samples were centrifuged (max. speed, 15 minutes, 4°C), supernatant was recovered and combined 1:4 with 50% methanol to obtain a final dilution of D20. |
Sample Type: | Bacterial cells |
Storage Conditions: | Described in summary |