Summary of Study ST002460
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001587. The data can be accessed directly via it's Project DOI: 10.21228/M8JX4P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002460 |
Study Title | Paleamon metabolomics |
Study Type | metabolomics |
Study Summary | The prawn Palaemon serratus exhibits a large distribution (occurring along the Northeastern Atlantic coast to the Mediterranean), and has thus been found suitable as model organism valuable for various ecotoxicological studies. However, little is still known about the potential input of its metabolome and particularly concerning a potential molecular sexual dimorphism observable in the different tissues of this organism. In an ecotoxicological point of view, inter-sex and inter-organ differences of the metabolomes may introduce analytical bias and impact the robustness of the analysis and its interpretation. To explore such possibilities, we obtained qualitative metabolomic data from the analysis of different organs of mature male and female Palaemon serratus. We used ultra-high-performance liquid chromatography-electrospray ionization-high resolution tandem mass spectrometry (UHPLC-ESI-HRMS on positive mode) to characterize the 75%-extracted metabolome of both gills, hepatopancreas, nervous gland, muscle and gonads. |
Institute | National Museum of Natural History |
Department | Aviv |
Laboratory | UMR 7245 MCAM |
Last Name | Marie |
First Name | Benjamin |
Address | 12 rue Buffon 75005 Paris - France |
bmarie@mnhn.fr | |
Phone | 331 40 79 32 12 |
Submit Date | 2023-01-26 |
Num Groups | 10 |
Total Subjects | 100 |
Num Males | 50 |
Num Females | 50 |
Raw Data Available | Yes |
Raw Data File Type(s) | mzXML |
Analysis Type Detail | LC-MS |
Release Date | 2023-02-15 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004014 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Bruker ELUTE UHPLC |
Column | Thermo Acclaim Polar Advantage II (150 x 3mm,3um) |
MS Type | ESI |
MS instrument type | QTOF |
MS instrument name | Bruker Compact |
Ion Mode | POSITIVE |
Units | counts |
Chromatography:
Chromatography ID: | CH002965 |
Chromatography Summary: | The metabolites were analysed by injection of 2 µL of the supernatants on an UHPLC (ELUTE, Bruker) using a Polar Advances II 2.5 pore C18 column (Thermo, Waltham, MA, USA) at a 300 µL·min−1 flow rate with a linear gradient of acetonitrile in 0.1% formic acid (5%–90% in 16 min). |
Instrument Name: | Bruker ELUTE UHPLC |
Column Name: | Thermo Acclaim Polar Advantage II (150 x 3mm,3um) |
Column Temperature: | 40 |
Flow Gradient: | 5-90% |
Flow Rate: | 0.3 mL/min |
Solvent A: | 100% water |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |