Summary of Study ST002460
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001587. The data can be accessed directly via it's Project DOI: 10.21228/M8JX4P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002460 |
| Study Title | Paleamon metabolomics |
| Study Type | metabolomics |
| Study Summary | The prawn Palaemon serratus exhibits a large distribution (occurring along the Northeastern Atlantic coast to the Mediterranean), and has thus been found suitable as model organism valuable for various ecotoxicological studies. However, little is still known about the potential input of its metabolome and particularly concerning a potential molecular sexual dimorphism observable in the different tissues of this organism. In an ecotoxicological point of view, inter-sex and inter-organ differences of the metabolomes may introduce analytical bias and impact the robustness of the analysis and its interpretation. To explore such possibilities, we obtained qualitative metabolomic data from the analysis of different organs of mature male and female Palaemon serratus. We used ultra-high-performance liquid chromatography-electrospray ionization-high resolution tandem mass spectrometry (UHPLC-ESI-HRMS on positive mode) to characterize the 75%-extracted metabolome of both gills, hepatopancreas, nervous gland, muscle and gonads. |
| Institute | National Museum of Natural History |
| Department | Aviv |
| Laboratory | UMR 7245 MCAM |
| Last Name | Marie |
| First Name | Benjamin |
| Address | 12 rue Buffon 75005 Paris - France |
| bmarie@mnhn.fr | |
| Phone | 331 40 79 32 12 |
| Submit Date | 2023-01-26 |
| Num Groups | 10 |
| Total Subjects | 100 |
| Num Males | 50 |
| Num Females | 50 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-02-15 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO002543 |
| Collection Summary: | Specimens of Palaemon serratus were collected by a professional fisherman (DYFLO) in February 2021, using special traps in the 2-nautical mile zone of the Cap of La Hève (Le Havre, Normandy, France). The collected prawns were returned to the laboratory within a maximum of 1 h, using 30 L-polypropylene tanks filled with artificial sea water (ASW; salinity of 33‰ from dissolution of TETRA SEA® salt, which is commonly used in maintenance of marine aquarium), in oxygenated conditions (Rollin et al., 2021). The prawns were transferred and kept into 80 L-aquariums that were connected to an ASW supply, and under constant aeration and filtration. Then, 10 mature males and gravid females (i.e. ovarian stage 4) of homogenous size (i.e. 8.5 ± 0.9 cm total length) and homogenous moult stage (i.e. D1’-D1’’) were anesthetized on ice and dissected. The gills, the nervous gland (i.e. eyestalks medulla and supra oesophageal ganglia), the muscle, the hepatopancreas and the gonads were weighted, individually frozen in liquid nitrogen and stored at -20°C. |
| Sample Type: | Shrimp organs |
| Collection Method: | Fishing net |
| Collection Location: | Le Havre, France |
| Storage Conditions: | -20℃ |
| Storage Vials: | eppendorfs |
