Summary of Study ST002474
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001597. The data can be accessed directly via it's Project DOI: 10.21228/M88H9Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002474 |
| Study Title | Retinol Dehydrogenase 10 Reduction Mediated Retinol Metabolism Disorder Promotes Diabetic Cardiomyopathy in Male Mice. |
| Study Summary | In this study, we identify disordered cardiac retinol metabolism in type 2 diabetic male mice and patients characterized by retinol overload, all-trans retinoic acid deficiency. By supplementing type 2 diabetic male mice with retinol or all-trans retinoic acid, we demonstrate that both cardiac retinol overload and all-trans retinoic acid deficiency promote diabetic cardiomyopathy. Mechanistically, by constructing cardiomyocyte-specific conditional retinol dehydrogenase 10-knockout male mice and overexpressing retinol dehydrogenase 10 in male type 2 diabetic mice via adeno-associated virus, we verify that the reduction in cardiac retinol dehydrogenase 10 is the initiating factor for cardiac retinol metabolism disorder and results in diabetic cardiomyopathy. Therefore, we suggest that the reduction of cardiac retinol dehydrogenase 10 and its mediated disorder of cardiac retinol metabolism is a new mechanism underlying diabetic cardiomyopathy. |
| Institute | Zhongshan School of Medicine, Sun Yat-sen University |
| Department | Dept. of Biochemistry |
| Last Name | Yandi |
| First Name | Wu |
| Address | 74, Zhongshan second street |
| wuyd3@mail2.sysu.edu.cn | |
| Phone | 15622158754 |
| Submit Date | 2023-02-08 |
| Publications | Retinol Dehydrogenase 10 Reduction Mediated Retinol Metabolism Disorder Promotes Diabetic Cardiomyopathy in Male Mice. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-02-13 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004041 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Sciex Triple Quad TM 4500MD MS |
| Column | Phenomenex Kinetex C18 (50 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTRAP |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | POSITIVE |
| Units | ng/ml |
Chromatography:
| Chromatography ID: | CH002989 |
| Instrument Name: | Sciex Triple Quad TM 4500MD MS |
| Column Name: | Phenomenex Kinetex C18 (50 x 2.1mm,1.7um) |
| Column Temperature: | 25 |
| Flow Gradient: | initial conditions 75% B; from 0 to 3.5min linear increase to 95% B; between 3.5 and 4.5min 95% B was retained; at 4.6 min back to initial conditions with 75% B; finally, 75% B was held from 4.6 to 5.9min |
| Flow Rate: | 0.2ml/min |
| Solvent A: | 40% acetonitrile/30% methanol/30% water; 0.1 % formic acid |
| Solvent B: | 55% acetonitrile/30% methanol/15% water; 0.1 % formic acid |
| Chromatography Type: | Reversed phase |
