Summary of Study ST002500

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001614. The data can be accessed directly via it's Project DOI: 10.21228/M82T5P This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002500
Study TitlePlasma metabolomic signatures from patients following high-dose total body irradiation
Study SummaryAlthough some progress has been made in the study of radiation injury, there are still no effective prevention and treatment methods for severe acute radiation syndrome or sickness (ARS). Accordingly, a thorough understanding of biological characteristics associated with high-dose radiation is essential for revealing the mechanisms underlying the varied biological processes following high dose radiation and the development of novel potent radioprotective agents. In present study, plasma metabolic characteristics were investigated from patients of hematopoietic stem cell transplantation following high-dose TBI pretreatment utilizing gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). The most potential panel of four metabolic markers for radiation injury was selected and the metabolic disorders involved were explored. The metabolic disorders implied the dysregulation of gut microflora, shift of energy supply from aerobic respiration to ketogenesis, protein synthesis and metabolism in response to TBI. Although similar metabolic alternation patterns exist between male and female following high-dose irradiation, specific changes are observed in either male or female patients. These findings provide valuable information for further selecting biomarkers, clues of pathogenic mechanisms involved in high-dose radiation exposure.
Institute
Soochow University
Last NameWang
First NameChang
AddressSuzhou, No. 199, Renai Road, Suzhou Industrial Park
Emailwangchang@suda.edu.cn
Phone+8651265880067
Submit Date2023-02-27
Raw Data AvailableYes
Raw Data File Type(s)cdf, raw(Thermo)
Analysis Type DetailGC-MS/LC-MS
Release Date2024-02-28
Release Version1
Chang Wang Chang Wang
https://dx.doi.org/10.21228/M82T5P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN004107 AN004108 AN004109
Analysis type MS MS MS
Chromatography type GC Reversed phase Reversed phase
Chromatography system Agilent 7890A Thermo TSQ Vantage HPLC-MS/MS Thermo TSQ Vantage HPLC-MS/MS
Column Agilent DB-5ms (30m x 0.25mm, 0.25um) Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um) Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
MS Type EI ESI ESI
MS instrument type Single quadrupole Triple quadrupole Triple quadrupole
MS instrument name Agilent 5977 Thermo TSQ Vantage Thermo TSQ Vantage
Ion Mode UNSPECIFIED POSITIVE NEGATIVE
Units pmoles/l pmoles/l pmoles/l

Chromatography:

Chromatography ID:CH003042
Instrument Name:Agilent 7890A
Column Name:Agilent DB-5ms (30m x 0.25mm, 0.25um)
Column Temperature:280
Flow Gradient:The oven temperature was initially set to 70°C, held for 3min, and then increased to 310°C at a rate of 5°C/min, and finally kept for 10min.
Flow Rate:1.2 mL/min
Solvent A:N/A
Solvent B:N/A
Chromatography Type:GC
  
Chromatography ID:CH003043
Chromatography Summary:positive ion mode
Instrument Name:Thermo TSQ Vantage HPLC-MS/MS
Column Name:Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um)
Column Temperature:50
Flow Gradient:0~1 min, 0.1~10% B; 1-5 min, 10-40% B; 5-17 min, 40-100% B; 17-22min, 100% B; 22-22.1 min, 100-10% B
Flow Rate:0.25 mL/min
Solvent A:water(0.1% formic acid )
Solvent B:acetonitrile(0.1% formic acid )
Chromatography Type:Reversed phase
  
Chromatography ID:CH003044
Chromatography Summary:negative ion mode
Instrument Name:Thermo TSQ Vantage HPLC-MS/MS
Column Name:Waters ACQUITY UPLC HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:50
Flow Gradient:0-1min,0.1%B;1-3min,0.1-40%B;3-5min,40-100%B;5-10.5min,100%B;10.5-10.6min,0.1%B;10.6-18min
Flow Rate:0.25 mL/min
Solvent A:water(5 mM ammonium bicarbonate )
Solvent B:95% methanol/water(5mM ammonium bicarbonate )
Chromatography Type:Reversed phase
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