Summary of Study ST002506

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001619. The data can be accessed directly via it's Project DOI: 10.21228/M8F41P This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002506
Study TitleNatural abundance of isotopic metabolite detection in mouse eye orgnaoids.
Study SummaryBecause the natural abundance of isotopic metabolites in the early eye organoids has not yet been reported, we have performed LC-MS/MS without exogenous isotopic labeling to show the natural abundance of isotopic carbons. Cell Name AES0145 : Rx-GFP K/I EB5 (RIKEN Cell Bank): Organoid method (PMID: 21475194).
Institute
Northwestern University
Last NameTAKATA
First NameNOZOMU
Address303 East Superior Street, 10-220
Emailnozomu.takata@northwestern.edu
Phone3125036066
Submit Date2023-03-15
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-05-01
Release Version1
NOZOMU TAKATA NOZOMU TAKATA
https://dx.doi.org/10.21228/M8F41P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN004128
Analysis type MS
Chromatography type HILIC
Chromatography system Q-exactive
Column Waters XBridge BEH Amide (100 x 3.0mm, 3.5um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode UNSPECIFIED
Units Peak area

Chromatography:

Chromatography ID:CH003058
Instrument Name:Q-exactive
Column Name:Waters XBridge BEH Amide (100 x 3.0mm, 3.5um)
Column Temperature:40
Flow Gradient:0 min, 15% A; 2.5 min, 30% A; 7 min, 43% A; 16 min, 62% A; 16.1-18 min, 75% A; 18-25 min, 15% A with a flow rate of 150 μL/min
Flow Rate:150 μL/mi
Solvent A:95% water/5% acetonitrile; 10 mM ammonium hydroxide; 10 mM ammonium acetate, pH 9.0
Solvent B:100% acetonitrile
Chromatography Type:HILIC
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