Summary of Study ST002507
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001619. The data can be accessed directly via it's Project DOI: 10.21228/M8F41P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002507 |
| Study Title | Time-course analysis of C13 labeling in mouse eye organoids. |
| Study Summary | The direct quantification of glucose consumption using 13C glucose time-course tracing was performed in cultured eye organoids and measured by LC-MS/MS analysis. The incubation was performed from 15 minutes to 2 hours. Cell Name AES0145 : Rx-GFP K/I EB5 (RIKEN Cell Bank): Organoid method (PMID: 21475194). |
| Institute | Northwestern University |
| Last Name | TAKATA |
| First Name | NOZOMU |
| Address | 303 East Superior Street, 10-220 |
| nozomu.takata@northwestern.edu | |
| Phone | 3125036066 |
| Submit Date | 2023-03-15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-06-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004129 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Q-exactive |
| Column | Waters XBridge BEH Amide (100 x 3.0mm, 3.5um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | UNSPECIFIED |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH003059 |
| Instrument Name: | Q-exactive |
| Column Name: | Waters XBridge BEH Amide (100 x 3.0mm, 3.5um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0 min, 15% A; 2.5 min, 30% A; 7 min, 43% A; 16 min, 62% A; 16.1-18 min, 75% A; 18-25 min, 15% A with a flow rate of 150 μL/min |
| Flow Rate: | 150 μL/mi |
| Solvent A: | 95% water/5% acetonitrile; 10 mM ammonium hydroxide; 10 mM ammonium acetate, pH 9.0 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
