Summary of Study ST002507

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001619. The data can be accessed directly via it's Project DOI: 10.21228/M8F41P This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002507
Study TitleTime-course analysis of C13 labeling in mouse eye organoids.
Study SummaryThe direct quantification of glucose consumption using 13C glucose time-course tracing was performed in cultured eye organoids and measured by LC-MS/MS analysis. The incubation was performed from 15 minutes to 2 hours. Cell Name AES0145 : Rx-GFP K/I EB5 (RIKEN Cell Bank): Organoid method (PMID: 21475194).
Northwestern University
First NameNOZOMU
Address303 East Superior Street, 10-220
Submit Date2023-03-15
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-06-08
Release Version1

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Sample Preparation:

Sampleprep ID:SP002613
Sampleprep Summary:The eye organoids were collected and washed twice with PBS before pellets were flash-frozen and stored at −80°C until metabolite extraction. Metabolite analysis was performed as described previously (PMID: 33931446). Briefly, for metabolite extraction, 80% methanol was used followed by the rapid freeze-thaw method to break the tissues. The supernatant underwent speedvac drying. The samples were prepared in 80% acetonitrile and were analyzed by High-Resolution Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS).