Summary of Study ST002511

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001621. The data can be accessed directly via it's Project DOI: 10.21228/M85M6X This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002511
Study TitleEnhanced niche colonization and competition during bacterial adaptation to a fungus
Study TypeFungal / bacterial interaction
Study SummaryEnhanced niche 1 colonization and competition during bacterial adaptation to a fungus
Institute
Netherlands Institute of Ecology
DepartmentMicrobial Ecology
Last NameTyc
First NameOlaf
AddressDroevendaalsesteeg 10, 6708 PB Wageningen, The Netherlands
EmailOlaf.Tyc@kgu.de
Phone+496963018046
Submit Date2023-03-16
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailGC-MS
Release Date2023-04-04
Release Version1
Olaf Tyc Olaf Tyc
https://dx.doi.org/10.21228/M85M6X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN004135
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890B
Column Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um)
MS Type EI
MS instrument type QTOF
MS instrument name Agilent 7200 QTOF
Ion Mode POSITIVE
Units m/z

Chromatography:

Chromatography ID:CH003063
Chromatography Summary:The volatiles were desorbed from the traps using an desorption unit (Unity TD-100, Markes International Ltd., Llantrisant, UK) at 210 °C for 12 min (He flow 50 mL/min) and trapped on a cold trap at -10 °C. The volatiles were introduced into the GC-Q-TOF (model Agilent 7890B GC and the Agilent 7200A QTOF, Santa Clara, USA) by heating the cold trap for 12 min to 250 °C. The used split ratio was 1:10 and the used column was a 30 × 0.25 mm ID RXI-5MS, film thickness 0.25 µm (Restek 13424-6850, Bellefonte, PA, USA). Temperature program was as follows: 39 °C for 2 min, from 39 °C to 95 °C at 3.5 °C/min, then to 165 °C at 4 °C/min, to 280 °C at 15 °C/min and finally to 320 °C at 30 °C/min, hold 7 min. The volatile organic compounds were ionized in EI mode at eV and mass spectra were acquired in full-scan-mode (30–400 U @ 5 scans/s). Mass-spectra were extracted with MassHunter Qualitative Analysis Software V B.06.00 Build 6.0.633.0 (Agilent Technologies, Santa Clara, USA).
Instrument Name:Agilent 7890B
Column Name:Restek Rtx-5Sil MS (30m x 0.25mm, 0.25um)
Column Temperature:280°C
Flow Gradient:-
Flow Rate:50 mL/min
Solvent A:-
Solvent B:-
Chromatography Type:GC
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