Summary of Study ST002511
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001621. The data can be accessed directly via it's Project DOI: 10.21228/M85M6X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002511 |
| Study Title | Enhanced niche colonization and competition during bacterial adaptation to a fungus |
| Study Type | Fungal / bacterial interaction |
| Study Summary | Enhanced niche 1 colonization and competition during bacterial adaptation to a fungus |
| Institute | Netherlands Institute of Ecology |
| Department | Microbial Ecology |
| Last Name | Tyc |
| First Name | Olaf |
| Address | Droevendaalsesteeg 10, 6708 PB Wageningen, The Netherlands |
| Olaf.Tyc@kgu.de | |
| Phone | +496963018046 |
| Submit Date | 2023-03-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | GC-MS |
| Release Date | 2023-04-04 |
| Release Version | 1 |
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Project:
| Project ID: | PR001621 |
| Project DOI: | doi: 10.21228/M85M6X |
| Project Title: | Enhanced niche colonization and competition during bacterial adaptation to a fungus |
| Project Type: | Fungal / bacterial interaction |
| Project Summary: | Bacterial-fungal interactions (BFIs) influence microbial community performance of most ecosystems and elicit specific microbial behaviors, including the stimulation of specialized metabolite production. Using a simple BFI system encompassing the Gram-positive bacterium Bacillus subtilis and the black mold fungus Aspergillus niger, we established a co-culture experimental evolution method to investigate bacterial adaptation to the presence of a fungus. In the evolving populations, B. subtilis was rapidly selected for enhanced production of the lipopeptide surfactin and accelerated surface spreading that led to an inhibition of fungal expansion and environment acidification. These phenotypes were explained by specific mutations in the DegS-DegU two-component system. In the presence of surfactin, the fungal hyphae exhibited bulged cells with delocalized secretory vesicles and RlmA-dependent cell wall stress induction. Increased surfactin production generally enhances the competitive success of the bacterium against various Aspergillus species that likely explains the primary adaption path in the presence of A. niger. |
| Institute: | Netherlands Institute of Ecology |
| Department: | Microbial Ecology |
| Laboratory: | GCMS Lab |
| Last Name: | Tyc |
| First Name: | Olaf |
| Address: | Theodor-Stern-Kai 7, Building 11, 60590 Frankfurt Germany |
| Email: | Olaf.Tyc@kgu.de |
| Phone: | 016094726403 |
